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GRF2 Antibody (OTI2F5) - Azide and BSA Free

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Western Blot: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunocytochemistry/ Immunofluorescence: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GRF2.
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70846AF350
Alexa Fluor 405
NBP2-70846AF405
Alexa Fluor 488
NBP2-70846AF488
Alexa Fluor 532
NBP2-70846AF532
Alexa Fluor 594
NBP2-70846AF594
Alexa Fluor 647
NBP2-70846AF647
Alexa Fluor 700
NBP2-70846AF700
Alexa Fluor 750
NBP2-70846AF750
Allophycocyanin
NBP2-70846APC
Biotin
NBP2-70846B
CoraFluor 1
NBP2-70846CL1
DyLight 350
NBP2-70846UV
DyLight 405
NBP2-70846V
DyLight 488
NBP2-70846G
DyLight 550
NBP2-70846R
DyLight 594
NBP2-70846DL594
DyLight 650
NBP2-70846C
DyLight 680
NBP2-70846FR
DyLight 755
NBP2-70846IR
FITC
NBP2-70846F
HRP
NBP2-70846H
Janelia Fluor 525
NBP2-70846JF525
Janelia Fluor 549
NBP2-70846JF549
Janelia Fluor 646
NBP2-70846JF646
Janelia Fluor 669
NBP2-70846JF669
PE
NBP2-70846PE
PerCP
NBP2-70846PCP
Unconjugated
NBP2-45515
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45515
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GRF2 Antibody (OTI2F5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAPGEF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:400-4000
Theoretical MW
122.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GRF2 Antibody (OTI2F5) - Azide and BSA Free

  • C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
  • CRK SH3-binding GNRP
  • DKFZp781P1719
  • Guanine nucleotide-releasing factor 2
  • Protein C3G
  • Rap guanine nucleotide exchange factor (GEF) 1
  • rap guanine nucleotide exchange factor 1

Background

GRF2 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GRF2 Antibody
(OTI2F5) - Azide and ...
NBP2-70846
GRF2 Antibody (OTI2F5) - Azide and BSA Free
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAPGEF1