![Western Blot: gp130/CD130 Antibody [NBP2-15776] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD130 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gp130-CD130-Antibody-Western-Blot-NBP2-15776-img0009.jpg "Western Blot: gp130/CD130 Antibody [NBP2-15776] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD130 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Rt, Mu, Po, Ca, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human gp130/CD130. The exact sequence is proprietary. |
| Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Secreted |
| Predicted Species | Mouse (91%), Porcine (95%), Rhesus Macaque (98%), Canine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IL6ST |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 104 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for gp130/CD130 Antibody (NBP2-15776)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: gp130/CD130 Antibody [NBP2-15776] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IL6ST protein stained by CD130 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/gp130-CD130-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15776-img0007.jpg "Immunocytochemistry/Immunofluorescence: gp130/CD130 Antibody [NBP2-15776] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IL6ST protein stained by CD130 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - Human breast carcinoma. CD130 stained by CD130 antibody diluted at 1:7500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/gp130-CD130-Antibody-Immunohistochemistry-Paraffin-NBP2-15776-img0010.jpg "Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - Human breast carcinoma. CD130 stained by CD130 antibody diluted at 1:7500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - J5 xenograft, using CD130 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp130-CD130-Antibody-Immunohistochemistry-Paraffin-NBP2-15776-img0004.jpg "Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - J5 xenograft, using CD130 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
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![N/A IL-11 [HRP]](https://images.novusbio.com/images2/DATA_IL11_D1100_ELISA_530.jpg)
![N/A IL-11 [HRP]](https://images.novusbio.com/images2/IL-11_D1100_ELISA_10.jpg)
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![N/A IL-6R alpha [HRP]](https://images.novusbio.com/images2/DATA_IL6_R_alpha_DR600_ELISA_801.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")