![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (GPC3/863) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-GPC3-863-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47762-img0006.jpg "Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (GPC3/863) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | GPC3/863 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44486). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Glypican 3 protein (Uniprot: P51654) |
| Localization | Cytoplasmic |
| Marker | Hepatocellular Carcinoma Marker |
| Specificity | Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GPC3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Glypican 3 Antibody (NBP2-47762)Find related products by research area.
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Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GPC3 |
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (GPC3/863)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-GPC3-863-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47762-img0007.jpg "Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (GPC3/863)")
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![Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Rat Heart stained with Glypican-3 Monoclonal Antibody (GPC3/863)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-GPC3-863-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47762-img0003.jpg "Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) - Azide and BSA Free [NBP2-47762] - Rat Heart stained with Glypican-3 Monoclonal Antibody (GPC3/863)")
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