Home » Endorepellin/Perlecan/Heparan Sulfate Proteoglycan » Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibodies » Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]
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Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]

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Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/ Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/ Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2364
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2364-SP
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Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endorepellin/Perlecan
Arg3684-Ser4391 (Ser4003Thr)
Accession # P98160
Specificity
Detects human Endorepellin/Perlecan in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HSPG2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2364 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]

  • basement membrane-specific heparan sulfate proteoglycan core protein
  • endorepellin (domain V region)
  • heparan sulfate proteoglycan 2
  • Heparan Sulfate Proteoglycan
  • HSPG
  • perlecan proteoglycan
  • perlecan
  • PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
  • PRCAN
  • SJA
  • SJS
  • SJS1

Background

Endorepellin is the C-terminal domain of the heparan sulfate proteoglycan perlecan, which is a modular protein found in basement membranes. Endorepellin has been found to be an inhibitor of angiogenesis that blocks endothelial cell adhesion to fibronectin and type I collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Endorepellin/Perlecan/ ...
AF2364
Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC

Publications for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (AF2364)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ICC
(2)
IHC
(1)
IHC-Fr
(1)
Western Blot
(2)
All Applications
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Human
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2364 Applications Species
Provenzale, I;De Simone, I;Gibbins, JM;Heemskerk, JWM;van der Meijden, PEJ;Jones, CI; Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan International journal of molecular sciences 2023-08-28 [PMID: 37686158] (ICC, Human) ICC Human
Y Xie, L He, R Lugano, Y Zhang, H Cao, Q He, M Chao, B Liu, Q Cao, J Wang, Y Jiao, Y Hu, L Han, Y Zhang, H Huang, L Uhrbom, C Betsholtz, L Wang, A Dimberg, L Zhang Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI Insight, 2021-08-09;0(0):. 2021-08-09 [PMID: 34228647] (IHC, Human) IHC Human
Malin Jansson, Ola Billing, Carl Herdenberg, Christina Lundin, Egle Tolockiene, Anoosheh Nazemroaya et al. Expression and Circulating Levels of Perlecan in Breast Cancer - Implications for Oestrogen Dependent Stromal Remodeling Journal of Mammary Gland Biology and Neoplasia 2020-03-01 [PMID: 32124140]
Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer, 2013-03-26;13(0):154. 2013-03-26 [PMID: 23530721] (ICC, IHC-Fr, Human) ICC, IHC-Fr Human
Parker TJ, Sampson DL, Broszczak D A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity. PLoS ONE, 2012-03-23;7(3):e33714. 2012-03-23 [PMID: 22457785] (Western Blot, Human) Western Blot Human
Goyal A, Pal N, Concannon M, Paul M, Doran M, Poluzzi C, Sekiguchi K, Whitelock JM, Neill T, Iozzo RV Endorepellin, the angiostatic module of perlecan, interacts with both the alpha2beta1 integrin and vascular endothelial growth factor receptor 2 (VEGFR2): a dual receptor antagonism. J. Biol. Chem., 2011-05-19;286(29):25947-62. 2011-05-19 [PMID: 21596751] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol HSPG2
Uniprot