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Glypican 4 Antibody

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Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody [NBP1-87697] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody [NBP1-87697] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human tonsil shows negative positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human kidney shows strong positivity in cytoplasm granular in cells in tubules.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human liver shows strong positivity in cytoplasm granular in hepatocytes.
Immunohistochemistry-Paraffin: Glypican 4 Antibody [NBP1-87697] - Staining of human pancreas shows strong positivity in cytoplasm granular in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Glypican 4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVR
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPC4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Glypican 4 Protein (NBP1-87697PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glypican 4 Antibody

  • Glypican 4
  • glypican proteoglycan 4
  • glypican-4
  • GPC4
  • K-glypican
  • K-glypicandJ900E8.1 (glypican 4)

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with avariable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family(GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. Theseproteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-87697
Glypican 4 Antibody
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GPC4