![Western Blot: Glypican 1 Antibody [NBP1-33197] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0030.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary. |
| Localization | Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Secreted glypican-1: Secreted, extracellular space |
| Predicted Species | Rhesus Macaque (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GPC1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Simple_Western_21377.jpg)
![Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Western_Blot_21459.jpg)
![Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Immunohistochemistry_17361.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GPC1 |
![Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33197-img0028.jpg "Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33197-img0029.jpg "Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Flow-Cytometry-NBP1-33197-img0017.jpg "Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0011.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0012.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0015.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0023.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody ] was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0025.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody ] was used to detect the primary antibody.")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0026.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0027.jpg "Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778")
![ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-ELISA-NBP1-33197-img0016.jpg "ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.")
![Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunoprecipitation-NBP1-33197-img0021.jpg "Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].")
![Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunoprecipitation-NBP1-33197-img0022.jpg "Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778")
![Western Blot Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Western_Blot_11618.jpg)
![Immunohistochemistry Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Immunohistochemistry_11640.jpg)
![Flow Cytometry Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Flow_Cytometry_11674.jpg)
![Immunocytochemistry Glypican 3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glypican_3_AF2119_Immunocytochemistry__Immunofluorescence_20870.jpg)
![Immunohistochemistry Glypican 3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glypican_3_AF2119_Immunohistochemistry_6734.jpg)
![Western Blot Glypican 3 Antibody [Unconjugated]](https://images.novusbio.com/images2/12878.jpg)
![Immunohistochemistry Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]](https://images.novusbio.com/images2/Endorepellin_AF2364_Immunohistochemistry_6744.jpg)
![Western Blot Decorin Antibody [Unconjugated]](https://images.novusbio.com/images2/Decorin_AF143_Western_Blot_17244.jpg)
![Simple Western Decorin Antibody [Unconjugated]](https://images.novusbio.com/images2/Decorin_AF143_Simple_Western_17469.jpg)
![Immunohistochemistry Decorin Antibody [Unconjugated]](https://images.novusbio.com/images2/Decorin_AF143_Immunohistochemistry_17623.jpg)
![Immunocytochemistry Versican Antibody [Unconjugated] - Isoform V0](https://images.novusbio.com/images2/Versican_AF3054_Immunocytochemistry_6488.jpg)
![Immunocytochemistry Versican Antibody [Unconjugated] - Isoform V0](https://images.novusbio.com/images2/af3054_human-versican-affinity-purified-polyclonal-ab-immunocytochemistry-842022131824.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")