![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; p<0.0001), middle (b; F(7,40)=33.89; p<0.0001), and caudal (c; F(7,40)=9.43; p<0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For each VMN segment, data depict mean normalized GAD O.D. valuesS.E.M. Results are summarized in the table at bottom. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; LZ = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glycogen phosphorylase- muscle form Antibody-Western Blot-NBP2-16689-img0008.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; p<0.0001), middle (b; F(7,40)=33.89; p<0.0001), and caudal (c; F(7,40)=9.43; p<0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For each VMN segment, data depict mean normalized GAD O.D. valuesS.E.M. Results are summarized in the table at bottom. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; LZ = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Ze, Bv, Rb, RM, ShSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glycogen phosphorylase, muscle form. The exact sequence is proprietary. |
| Predicted Species | Bovine (96%), Rabbit (95%), Rhesus Macaque (98%), Sheep (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PYGM |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 97 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
![Immunohistochemistry ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Immunohistochemistry_6703.jpg)
![Western Blot ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Flow_Cytometry_20028.jpg)
![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Human breast cancer. PYGM antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Immunohistochemistry-Paraffin-NBP2-16689-img0007.jpg "Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Human breast cancer. PYGM antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Western-Blot-NBP2-16689-img0006.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-western-blot-2762023105814..jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-western-blot-27620231106..jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse muscle. PYGM stained by PYGM antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-immunohistochemistry-paraffin-276202311154..jpg "Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse muscle. PYGM stained by PYGM antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-immunohistochemistry-paraffin-276202311258..jpg "Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] at 1:300 dilution.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-310202415175293.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-31020241534310.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-310202416121962.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated]](https://images.novusbio.com/images2/FUBI_MAB9036_Immunocytochemistry__Immunofluorescence_19164.jpg)
![Western Blot FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated]](https://images.novusbio.com/images2/FUBI_MAB9036_Western_Blot_19167.jpg)
![Immunohistochemistry Ret Antibody [Unconjugated]](https://images.novusbio.com/images2/Ret_AF482_Immunohistochemistry_10194.jpg)
![Western Blot Ret Antibody [Unconjugated]](https://images.novusbio.com/images/af482_mouse-ret-affinity-purified-polyclonal-ab-8220249503265.jpg)
![Immunohistochemistry Ret Antibody [Unconjugated]](https://images.novusbio.com/images/af482_mouse-ret-affinity-purified-polyclonal-ab-8220249505961.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")