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Deoxyguanosine kinase Antibody

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Western Blot: Deoxyguanosine kinase Antibody [NBP1-84256] - Analysis in control (vector only transfected HEK293T lysate) and DGUOK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in ...read more
Immunohistochemistry-Paraffin: Deoxyguanosine kinase Antibody [NBP1-84256] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Deoxyguanosine kinase Antibody [NBP1-84256] - Staining of human Lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Deoxyguanosine kinase Antibody [NBP1-84256] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Deoxyguanosine kinase Antibody [NBP1-84256] - Staining of human Testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Deoxyguanosine kinase Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: STFVKLLTKTYPEWHVATEPVATWQNIQAAGTQKACTAQSLGNLLDMMYREPARWSYTFQTFSFLSRLKVQLEPFPEKLLQARKPVQI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGUOK
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Deoxyguanosine kinase Protein (NBP1-84256PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Deoxyguanosine kinase Antibody

  • deoxyguanosine kinase
  • deoxyguanosine kinase, mitochondrial
  • DGK
  • dGKmitochondrial deoxyguanosine kinase
  • EC 2.7.1.113
  • MTDPS3

Background

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DGUOK