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Recombinant Human FGF-3 Protein

 (14 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
1206-F3-025/CF
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Recombinant Human FGF-3 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.02-0.1 µg/mL in the presence of 1 µg/mL of heparin.
Source
E. coli-derived human FGF-3 protein
Asp28-Arg212, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1206-F3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-3 Protein

  • FGF3
  • FGF-3
  • fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog)
  • fibroblast growth factor 3
  • HBGF-3
  • Hst
  • INT-2
  • mouse
  • oncogene INT2

Background

Fibroblast Growth Factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

The FGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration (2). Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site (1). Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus (5). FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity (6). FGF-3 has been implicated in the induction of inner ear development (7). Studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation (8 - 10). During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by Sef (similar expression to fgf genes), a transmebrane protein that shares intracellular sequence similarities with the IL-17 receptor (10).

  1. Brookes, S. et al. (1989) Oncogene 4:429.
  2. Dickson, C. et al. (1989) Prog. Growth Factor Res. 1:123.
  3. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311. 
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Antoine, M. et al. (2000) Cell Growth Differen. 11:593.
  6. Kohl, R. et al. (2002) J. Biol. Chem. 277:32760.
  7. Represa, J. et al. (1991) Nature 353:561.
  8. Maroon, H. et al. (2002) Development, 129:2099.
  9. Walshe, J. et al. (2002) Current Biol. 12:1117.
  10. Furthauer, M. et al. (2002) Nature Cell Biol. 4:170.

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Publications for FGF-3 (1206-F3)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Zebrafish.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(12)
Cell Culture
(1)
In Vivo
(1)
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Human
(10)
Mouse
(2)
Chicken
(1)
Zebrafish
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 1206-F3 Applications Species
Chen, Y;Mu, W;Wu, Y;Xu, J;Li, X;Hu, H;Wang, S;Wang, D;Hui, B;Wang, L;Dong, Y;Chen, W; Optogenetically modified human embryonic stem cell-derived otic neurons establish functional synaptic connection with cochlear nuclei Journal of tissue engineering 2024-07-31 [PMID: 39092452] (Bioassay, Human) Bioassay Human
Kømurcu, KS;Wilhelmsen, I;Thorne, JL;Krauss, S;Wilson, SR;Aizenshtadt, A;Røberg-Larsen, H; Mass Spectrometry Reveals that Oxysterols are Secreted from Non-Alcoholic Fatty Liver Disease Induced Organoids The Journal of steroid biochemistry and molecular biology 2023-06-26 [PMID: 37380087] (Bioassay, Human) Bioassay Human
T Saeki, S Yoshimatsu, M Ishikawa, CC Hon, I Koya, S Shibata, M Hosoya, C Saegusa, K Ogawa, JW Shin, M Fujioka, H Okano Critical roles of FGF, RA, and WNT signalling in the development of the human otic placode and subsequent lineages in a dish Regenerative Therapy, 2022-05-16;20(0):165-186. 2022-05-16 [PMID: 35620640] (Bioassay, Human) Bioassay Human
L Cui, J Zheng, Q Zhao, JR Chen, H Liu, G Peng, Y Wu, C Chen, Q He, H Shi, S Yin, RA Friedman, Y Chen, MX Guan Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss JCI Insight, 2020-12-03;5(23):. 2020-12-03 [PMID: 33268592] (Bioassay, Human) Bioassay Human
SL Boddy, R Romero-Gue, AR Ji, C Unger, L Corns, W Marcotti, MN Rivolta Generation of Otic Lineages from Integration-Free Human-Induced Pluripotent Stem Cells Reprogrammed by mRNAs Stem Cells Int, 2020-03-01;2020(0):3692937. 2020-03-01 [PMID: 32190057] (Bioassay, Human) Bioassay Human
S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019-06-11;9(1):8470. 2019-06-11 [PMID: 31186471] (Bioassay, Human) Bioassay Human
H Lahlou, A Lopez-Juar, A Fontbonne, E Nivet, A Zine Modeling human early otic sensory cell development with induced pluripotent stem cells PLoS ONE, 2018-06-14;13(6):e0198954. 2018-06-14 [PMID: 29902227] (Bioassay, Human) Bioassay Human
C Zhong, Z Chen, X Luo, C Wang, H Jiang, J Shao, M Guan, L Huang, X Huang, J Wang Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells Int. J. Biochem. Cell Biol., 2018-02-06;96(0):79-89. 2018-02-06 [PMID: 29413750] (Cell Culture, Mouse) Cell Culture Mouse
AB Hanker, JG Garrett, MV Estrada, PD Moore, PG Ericsson, JP Koch, E Langley, S Singh, PS Kim, GM Frampton, EM Sanford, P Owns, J Becker, MR Groseclose, S Castellino, H Joensuu, J Huober, JC Brase, M Samira, S Brohée, D Venet, D Brown, J Baselga, M Piccart, C Sotiriou, CL Arteaga HER2-overexpressing breast cancers amplify FGFR signaling upon acquisition of resistance to dual therapeutic blockade of HER2 Clin. Cancer Res., 2017-04-05;0(0):. 2017-04-05 [PMID: 28381415] (Bioassay, Human) Bioassay Human
Dalle Nogare D, Somers K, Rao S, Matsuda M, Reichman-Fried M, Raz E, Chitnis A Leading and trailing cells cooperate in collective migration of the zebrafish posterior lateral line primordium. Development, 2014-07-25;141(16):3188-96. 2014-07-25 [PMID: 25063456] (In Vivo, Zebrafish) In Vivo Zebrafish
Show All 14 Publications.

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Bioinformatics

Gene Symbol FGF3
Uniprot