We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

I want to know why GAPDH is used as a loading control antibody in WB techniques.

Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?

Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

What secondary antibody should I use for visualization of the GAPDH antibody?

This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

Do you offer a smaller size for the GAPDH antibodies?

We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

For homogenized tissue, beta-actin and GAPDH are both fine.

We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.

GAPDH Antibody (686613) [Unconjugated] (MAB5718): Novus Biologicals

Name: GAPDH Antibody (686613) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB5718
Rating: 5 (1 reviews)

- COVID-19 Research Tools
  
  Antibodies
  Primary Antibodies
  Conjugated Antibodies
  
  Sample Size Antibodies
  
  KO Validated Antibodies
  
  Recombinant Antibodies
  
  Antibody Sampler Packs
  
  Antibody Pairs
  
  Secondary Antibodies
  
  Isotype Control Antibodies
  
  Proteins and Peptides
  Peptides & Proteins
  
  Blocking Peptides
  
  Biologically Active Proteins
  
  Lysates and Cell Lines
  Lysates
  
  Stem Cell Lines
- Kits
  Antibody Labeling Kits
  
  ChIP Kits
  
  ELISA Kits
  
  Flow Cytometry Kits
  
  Activators and Inhibitors
  Inhibitors
  
  Activators
  
  Support Products
  Exosome Isolation
  
  Flow Cytometry Beads
  
  IHC Tissue Slides
  
  IHC Microarrays
  
  Western Blot Gels & Buffers
  
  Western Blot Membranes
  
  All Support Products
  
  Bulk and Custom
Products
Our Protocols
- Resources
  Protocols
  
  Video Protocols
  
  Troubleshooting Guides
  
  Posters and Literature
  
  Handbooks and Guides
  
  Calculators
  
  FAQs
  
  Blogs
  
  Diseases
  
  Whitepapers
  
  Antibody Concierge
  
  Webinars
- Tools
  Spectra Viewer
  
  Flow Cytometry Panel Builder
  
  Application
  ELISA
  
  Flow Cytometry
  
  Mass Cytometry (CyTOF)
  
  ICC
  
  IHC
  
  Western Blotting
  
  Interviews
  Faculty
  
  Postdoc
Resources
View Pathways
- By Research Area
  Apoptosis
  
  Autophagy
  
  Cancer
  
  Cell Biology
  
  Cellular Markers
  
  Epigenetics
  
  Epitope Tags
  
  Exosomes Research
  
  Hypoxia
  
  Immunology
  
  Microbiology
  
  Neuroscience
  
  Stem Cells
- By Pathway
  AKT
  
  Apoptosis
  
  Autophagy
  
  ERK1/2/5 Pathway
  
  Hedgehog
  
  Hippo
  
  JNK Pathway
  
  mTOR
  
  Necroptosis
  
  NFKB Signaling
  
  p38 Signaling
  
  TGF Beta
  
  TLR
Research Areas
Get Rewards
- Customer Care
  100% Guarantee
  
  Risk Free Testing
  
  Novus Rewards Program
  
  Publication Rewards Program
  
  New Lab Discount
  
  Scholarship
  
  Travel Grants
  
  Promotions
Customer Care
Novus Careers
- About us
  Careers
  
  Collaborations
  
  Events
  
  Novus Story
  
  Our Team
  
  Philanthropy
  
  Press Releases
  
  Why choose Novus
About Us
- Contact Us
  Customer Service
  
  Distributors
  
  Technical Support
Contact

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Simple Western, IHC, ICC/IF
Clone	686613
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Catalog# & Formulation	Size	Price
Carrier Free MAB5718-SP

Immunogen	E. coli-derived recombinant human GAPDH/G3PDH Met1-Ala150 Accession # P04406
Specificity	Detects human, mouse and rat GAPDH/G3PDH in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse GAPDH/G3PDH and no cross-reactivity with recombinant human GAPDH-2 is observed.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	GAPDH
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Reconstitution Instructions	Sterile PBS to a final concentration of 0.5 mg/mL.

GAPDH Antibody (686613) [Unconjugated]

Species Glossary

Images

Product Details

Order Details

GAPDH Antibody (686613) [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for GAPDH Antibody (686613) [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for GAPDH Antibody (MAB5718)(41)

Review for GAPDH Antibody (MAB5718) (1) 51

Summary

Product General Protocols

Video Protocols

FAQs for GAPDH Antibody (MAB5718). (Showing 1 - 7 of 7 FAQs).

Secondary Antibodies

Isotype Controls

Additional GAPDH Products

Blogs on GAPDH. Showing 1-10 of 13 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Publications using MAB5718	Applications	Species
Jeanae M. Kaneshiro, Juliana S. Capitanio, Martin W. Hetzer Lamin B1 overexpression alters chromatin organization and gene expression Nucleus 2023-12-31 [PMID: 37071033]
H Kim, HJ Park, HW Chang, JH Back, SJ Lee, YE Park, EH Kim, Y Hong, G Kwak, IC Kwon, JE Lee, YS Lee, SY Kim, Y Yang, SH Kim Exosome-guided direct reprogramming of tumor-associated macrophages from protumorigenic to antitumorigenic to fight cancer Bioactive materials, 2022-08-05;25(0):527-540. 2022-08-05 [PMID: 37056267]
AN Gonçalves, RS Moura, J Correia-Pi, C Nogueira-S Intraluminal chloride regulates lung branching morphogenesis: involvement of PIEZO1/PIEZO2 Respiratory Research, 2023-02-05;24(1):42. 2/5/2023 [PMID: 36740669]
S Mohammadho, A Weaver, M Sudhakaran, LC Ho, T Le, AI Doseff, F Medina-Bol Arachidin-1, a Prenylated Stilbenoid from Peanut, Enhances the Anticancer Effects of Paclitaxel in Triple-Negative Breast Cancer Cells Cancers, 2023-01-07;15(2):. 2023-01-07 [PMID: 36672351] (Western Blot, Human)	Western Blot	Human
Shinji Sugita, Yoshifumi Naito, Li Zhou, Hongli He, Qi Hao, Atsuhiro Sakamoto et al. Hyaluronic acid restored protein permeability across injured human lung microvascular endothelial cells FASEB BioAdvances 2022-09-01 [PMID: 36089980]
Claire W. Chang, Ni Cheng, Yanyan Bai, Randal A. Skidgel, Xiaoping Du G alpha 13 Mediates Transendothelial Migration of Neutrophils by Promoting Integrin-Dependent Motility without Affecting Directionality The Journal of Immunology 2021-12-15 [PMID: 34799423]
Mariko Sawa, Cassia Overk, Ann Becker, Dominique Derse, Ricardo Albay, Kim Weldy et al. Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model Alzheimer's & Dementia 2022-06-01 [PMID: 34757693]
Vanessa de Oliveira Furino, João Manoel Alves, Diego Adorna Marine, Marcela Sene-Fiorese, Carla Nascimento dos Santos Rodrigues, Cristina Arrais-Lima et al. Dietary Intervention, When Not Associated With Exercise, Upregulates Irisin/FNDC5 While Reducing Visceral Adiposity Markers in Obese Rats Frontiers in Physiology 2021-08-13 [PMID: 34483949]
Polona Safaric Tepes, Debjani Pal, Trine Lindsted, Ingrid Ibarra, Amaia Lujambio, Vilma Jimenez Sabinina et al. An epigenetic switch regulates the ontogeny of AXL-positive/EGFR-TKi-resistant cells by modulating miR-335 expression eLife 2021-07-13 [PMID: 34254585]
Stephen Gargan, Paul Dowling, Margit Zweyer, Jens Reimann, Michael Henry, Paula Meleady et al. Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy Life (Basel) 2021-06-22 [PMID: 34206383]
F Schneider, I Metz, S Khudayberd, MB Rust Functional Redundancy of Cyclase-Associated Proteins CAP1 and CAP2 in Differentiating Neurons Cells, 2021-06-17;10(6):. 2021-06-17 [PMID: 34204261] (Western Blot, Mouse)	Western Blot	Mouse
Presa M, Bailey RM, Davis C et al. AAV9-mediated FIG4 delivery prolongs life span in Charcot Marie Tooth disease type 4J mouse model The Journal of clinical investigation 4/20/2021 [PMID: 33878035]
LJ Kepser, S Khudayberd, LS Hinojosa, C Macchi, M Ruscica, E Marcello, C Culmsee, R Grosse, MB Rust Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts Scientific Reports, 2021-02-26;11(1):4789. 2021-02-26 [PMID: 33637797] (Western Blot, Mouse)	Western Blot	Mouse
Lior Soday, Martin Potts, Leah M. Hunter, Benjamin J. Ravenhill, Jack W. Houghton, James C. Williamson et al. Comparative Cell Surface Proteomic Analysis of the Primary Human T Cell and Monocyte Responses to Type I Interferon Frontiers in Immunology 2021-02-08 [PMID: 33628210]
Kui Li, Zheng Zhou, Ju Li, Rui Xiang miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1 Technol Cancer Res Treat 2020-12-17 [PMID: 33327874]
J Fisher, OE Sørensen, AHA Abu-Humaid Using chemiluminescence imaging of cells (CLIC) for relative protein quantification Sci Rep, 2020-10-26;10(1):18280. 2020-10-26 [PMID: 33106566] (ICC, Human)	ICC	Human
Liu WH, Mrozek-Gorska P, Wirth AK et al. Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL Biomarker research 9/16/2020 [PMID: 32944247]
Z Li, J Zheng, D Wan, X Yang Uniaxial Static Strain Promotes Osteoblast Proliferation and Bone Matrix Formation in Distraction Osteogenesis In Vitro Biomed Res Int, 2020-08-12;2020(0):3906426. 2020-08-12 [PMID: 32855965] (Western Blot, Rat)	Western Blot	Rat
E Cienfuegos, TR Ibarra-Riv, AL Saucedo, LA Ramírez-Ma, D Esquivel-F, I Domínguez-, KJ Alcántara-, DP Moreno-Peñ, G Alarcon-Ga, DR Rodríguez-, L Torres-Gon, LE Muñoz-Espi, E Pérez-Rodr, P Cordero-Pé Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway PeerJ, 2020-07-10;8(0):e9438. 2020-07-10 [PMID: 32728491] (Western Blot, Rat)	Western Blot	Rat
Hao Dong, Fengming Zhu, Shilu Jin, Jing Tian Interleukin‑22 regulates gastric cancer cell proliferation through regulation of the JNK signaling pathway Experimental and Therapeutic Medicine 2020-04-30 [PMID: 32536992]
G Kaufman, NM Kiburi, D Skrtic The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer Biosci. Rep., 2020-06-26;40(6):. 2020-06-26 [PMID: 32495822] (ICC, Human)	ICC	Human
KV Lithgow, B Church, A Gomez, E Tsao, S Houston, LA Swayne, CE Cameron Identification of the Neuroinvasive Pathogen Host Target, LamR, as an Endothelial Receptor for the Treponema pallidum Adhesin Tp0751 mSphere, 2020-04-01;5(2):. 2020-04-01 [PMID: 32238570] (Co-Immunoprecipitation, Human)	Co-Immunoprecipitation	Human
Nobre LV, Nightingale K, Ravenhill BJ et al. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions Elife 12/24/2019 [PMID: 31873071]
Yeonsun Hong, Yoon Kyoung Kim, Gi Beom Kim, Gi-Hoon Nam, Seong A Kim, Yoon Park et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity Journal of Extracellular Vesicles 2019-12-01 [PMID: 31632619]
S Varshney, HX Wei, F Batista, M Nauman, S Sundaram, K Siminovitc, A Tanwar, P Stanley A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice BMC Dev. Biol., 2019-10-07;19(1):19. 2019-10-07 [PMID: 31590629] (Western Blot, Mouse)	Western Blot	Mouse
László-István Bába, Melinda Kolcsár, Imre Zoltán Kun, Zsófia Ulakcsai, Fruzsina Bagaméry, Éva Szökő et al. Effects of Cariprazine, Aripiprazole, and Olanzapine on Mouse Fibroblast Culture: Changes in Adiponectin Contents in Supernatants, Triglyceride Accumulation, and Peroxisome Proliferator-Activated Receptor-gamma Expression Medicina (Kaunas) 2019-05-17 [PMID: 31108997]
K Watanabe, M Suzukawa, S Arakawa, K Kobayashi, S Igarashi, H Tashimo, H Nagai, S Tohma, T Nagase, K Ohta Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor Allergol Int, 2019-04-24;0(0):. 2019-04-24 [PMID: 31029506] (Western Blot, Human)	Western Blot	Human
RM Fliefel, SA Entekhabi, M Ehrenfeld, S Otto Geranylgeraniol (GGOH) as a Mevalonate Pathway Activator in the Rescue of Bone Cells Treated with Zoledronic Acid: An In Vitro Study Stem Cells Int, 2019-01-09;2019(0):4351327. 2019-01-09 [PMID: 30728841] (Western Blot, Human)	Western Blot	Human
A Sharma, S Bandyopadh, K Chowdhury, T Sharma, R Maheshwari, A Das, G Chakrabart, V Kumar, CC Mandal Metformin exhibited anticancer activity by lowering cellular cholesterol content in breast cancer cells PLoS ONE, 2019-01-09;14(1):e0209435. 2019-01-09 [PMID: 30625181] (Western Blot, Human)	Western Blot	Human
Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway Medical Science Monitor 2018-12-15 [PMID: 30552850]
Katie Nightingale, Kai-Min Lin, Benjamin J. Ravenhill, Colin Davies, Luis Nobre, Ceri A. Fielding et al. High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms Cell Host & Microbe 2018-09-12 [PMID: 30122656]
Angelika Schmitt, Anne-Lena Haug, Franziska Schlegel, Annunziata Fragasso, Barbara Munz Effects of 10 weeks of regular running exercise with and without parallel PDTC treatment on expression of genes encoding sarcomere-associated proteins in murine skeletal muscle Cell Stress and Chaperones 2018-09-01 [PMID: 29797237]
SY Ma'ayeh, J Liu, D Peirasmaki, K Hörnaeus, S Bergström, M Grabherr, J Bergquist, SG Svärd Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells PLoS Negl Trop Dis, 2017-12-11;11(12):e0006120. 2017-12-11 [PMID: 29228011] (Western Blot, Human)	Western Blot	Human
Lijie Zhao, Tingli Sun, Lina Wang Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus Therapeutics and Clinical Risk Management 2017-07-20 [PMID: 28721055]
Zhou Yan-Wu, Li Rong, Duan Chao-Jun et al. Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma. Molecular Medicine Reports 1/1/2017 [PMID: 28000881]
Feng Xu, Ling-Yun Wu, Chun-Kang Chang, Qi He, Zheng Zhang, Li Liu et al. Whole-exome and targeted sequencing identify ROBO1 and ROBO2 mutations as progression-related drivers in myelodysplastic syndromes Nature Communications 2015-11-26 [PMID: 26608094]
Gutknecht M, Geiger J, Joas S, Dorfel D, Salih H, Muller M, Grunebach F, Rittig S The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells. Cell Commun Signal, 2015-03-24;13(0):19. 2015-03-24 [PMID: 25889792] (Western Blot, Human)	Western Blot	Human
Stanley Tamaki, Christopher Nye, Euan Slorach, David Scharp, Helen M Blau, Phyllis E Whiteley et al. Simultaneous silencing of multiple RB and p53 pathway members induces cell cycle reentry in intact human pancreatic islets BMC Biotechnology 2014-12-01 [PMID: 25305068]
Yue Chen, Zhiqiang Zheng, Xi Zhu, Yujie Shi, Dandan Tian, Fengjuan Zhao et al. Lactoferrin Promotes Early Neurodevelopment and Cognition in Postnatal Piglets by Upregulating the BDNF Signaling Pathway and Polysialylation Molecular Neurobiology 2015-08-01 [PMID: 25146846]
Kondo S, Mukudai Y, Soga D, Nishida T, Takigawa M, Shirota T Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma. Anticancer Res, 2014-02-01;34(2):671-7. 2014-02-01 [PMID: 24510998] (Western Blot, Human)	Western Blot	Human
Liang G, Gao W, Liang A Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis. PLoS ONE, 2012-05-15;7(5):e36648. 2012-05-15 [PMID: 22615788] (Western Blot, Human)	Western Blot	Human
Show All 41 Publications. Collapse Publications.

	Verified Customer 02/25/2015
Application:	WB
Species:	Mouse

Gene Symbol	GAPDH
Uniprot	P04406(Human)