![Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NB100-40825-img0006.jpg "Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1160 and the C-terminus (residue 1210) of human G9A (Euchromatic Histone-Lysine N-methyltransferase 2) using the numbering given in entry NP_006700.2 (GeneID 10919). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EHMT2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | While trace amounts of G9A/EHMT2 can be immunoprecipitated by this antibody, the efficiency is very low. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 01/26/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for G9a/EHMT2 Antibody (NB100-40825)Find related products by research area.
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![Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Immunohistochemistry-NB100-40825-img0007.jpg "Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Immunoprecipitation-NB100-40825-img0008.jpg "Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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