![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-NBP1-21367-img0006.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
| Reactivity | Hu, Mu, Bv, Pm-OrSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human KMT1A/SUV39H1 protein (between residues 1-100) [UniProt O43463] |
| Localization | Nucleus. |
| Predicted Species | Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SUV39H1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC-P, WB reactivitiy reported in scientific literature (PMID: 23943221), and verified customer reviews. Use in ICC/IF reported in scientific literature (PMID:28425504). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
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WB | Human | 01/29/2014 |
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WB | Human | 01/29/2014 |
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IHC-P | Human | 01/29/2014 |
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IHC-P | Human | 01/29/2014 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for KMT1A/SUV39H1 Antibody (NBP1-21367)Find related products by research area.
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| Application: | WB | |
| Species: | Human |
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| Application: | WB | |
| Species: | Human |
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| Application: | IHC-P | |
| Species: | Human |
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP1-21367-img0003.jpg "Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.")
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0004.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.")
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0005.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
