![Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Western-Blot-NBP2-20322-img0009.jpg "Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (90%), Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SETDB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 143 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
| Publications using NBP2-20322 | Applications | Species |
|---|---|---|
| Lin J, Guo D, Liu H et al. The SETDB1-TRIM28 Complex Suppresses Antitumor Immunity Cancer Immunology Research 2021-12-01 [PMID: 34848497] (Immunohistochemistry) | Immunohistochemistry | |
| Roy A, Ghosh A, Kumar B, Chandran B IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP Elife 2019-11-04 [PMID: 31682228] (WB, IP, Chemotaxis, ICC/IF, PLA, Human) | WB, IP, Chemotaxis, ICC/IF, PLA | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
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WB | Human | 06/18/2018 |
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reviewed by:
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ICC | Human | 07/12/2017 |
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IP | Human | 04/20/2017 |
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reviewed by:
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WB | Human | 04/19/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for SETDB1 Antibody (NBP2-20322)Find related products by research area.
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| Verified Customer 06/18/2018 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 07/12/2017 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 04/20/2017 |
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| Application: | IP | |
| Species: | Human |
![Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunocytochemistry-NBP2-20322-img0006.jpg "Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20322-img0007.jpg "Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-20322-img0003.jpg "Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.")
![Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunoprecipitation-NBP2-20322-img0005.jpg "Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![ICC/IF-SETDB1 Antibody [NBP2-20322] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp2-20322_rabbit-polyclonal-setdb1-antibody-immunocytochemistry-immunofluorescence-8122023143536..jpg "ICC/IF-SETDB1 Antibody [NBP2-20322] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10um.")
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![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
![N/A alpha-Defensin 1 [Biotin]](https://images.novusbio.com/images2/DATA_alphaDefensin_1_DY819805_ELISA_2534.jpg)
![GLP/EHMT1 Antibody (B0422) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GLP/EHMT1 Antibody (B0422) [Unconjugated]")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
