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![Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NB100-40825-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Immunohistochemistry-NB100-40825-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Immunoprecipitation-NB100-40825-img0008.jpg)
![Western Blot: G9a/EHMT2 Antibody (SR1220) [NBP3-22401] - Western blot analysis using NBP3-22401 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22401_rabbit-g9a-ehmt2-mab-sr1220-57202315584820.jpg "Western Blot: G9a/EHMT2 Antibody (SR1220) [NBP3-22401] - Western blot analysis using NBP3-22401 in 293 cell lysate.")
![Western Blot: G9a/EHMT2 Antibody [NBP2-55881] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NBP2-55881-img0002.jpg "Western Blot: G9a/EHMT2 Antibody [NBP2-55881] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55881-img0001.jpg)
![Western Blot: EHMT2 Overexpression Lysate (Adult Normal) [NBL1-10164] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EHMT2.](http://images.novusbio.com/fullsize/EHMT2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10164-img0002.jpg "Western Blot: EHMT2 Overexpression Lysate (Adult Normal) [NBL1-10164] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EHMT2.")
![Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2 , diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NBP3-18664-img0001.jpg "Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2 , diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.")
![ELISA: G9a/EHMT2 Antibody [NBP3-18664] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a/EHMT2 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.](http://images.novusbio.com/fullsize/G9a-EHMT2 Antibody-ELISA-NBP3-18664-img0002.jpg)
![Western Blot: G9a/EHMT2 Antibody (SR1247) [NBP3-22088] - Western blot analysis using NBP3-22088 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22088_rabbit-g9a-ehmt2-mab-sr1247-5720231552187.jpg "Western Blot: G9a/EHMT2 Antibody (SR1247) [NBP3-22088] - Western blot analysis using NBP3-22088 in HeLa cell lysate.")
![Western Blot: G9a/EHMT2 Antibody [NBP2-42846] - Analysis of 30 ug whole cell lysate. A:NIH-3T3 5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NBP2-42846-img0001.jpg "Western Blot: G9a/EHMT2 Antibody [NBP2-42846] - Analysis of 30 ug whole cell lysate. A:NIH-3T3 5% SDS PAGE diluted at 1:1000.")
![Chromatin Immunoprecipitation: G9a/EHMT2 Antibody [NBP3-10942] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Chromatin-Immunoprecipitation-NBP3-10942-img0001.jpg "Chromatin Immunoprecipitation: G9a/EHMT2 Antibody [NBP3-10942] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.")