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EZH2/KMT6 Antibody (CL11915)

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Analysis in human testis and cerebral cortex tissues using NBP3-21204 antibody. Corresponding EZH2 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Analysis in human testis and cerebral cortex tissues using NBP3-21204 antibody. Corresponding EZH2 RNA-seq data are presented for the same tissues.
Staining of U-251 cells using the Anti-EZH2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.
Staining of human kidney shows mainly absence of positivity in cells in tubules as expected.
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Staining of human glioblastoma multiforme shows strong nuclear positivity in tumor cells.
Analysis in human cell line MOLT-4.
Staining of human oligodendroglioma shows strong nuclear positivity in tumor cells.
Staining of human lymph node shows moderate to strong nuclear positivity in lymphoid cells.
Staining of human cerebral cortex shows no positivity in neurons as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL11915
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

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EZH2/KMT6 Antibody (CL11915) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of Q15910. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EZH2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for EZH2/KMT6 Antibody (CL11915)

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

KMT6 / EZH2 encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EZH2/KMT6 Antibody
(CL11915)
NBP3-21204
EZH2/KMT6 Antibody (CL11915)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Bioinformatics

Gene Symbol EZH2