![Western Blot: Recombinant Human Enolase 1 Protein [NBP1-30262] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Enolase 1 antibody [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.](http://images.novusbio.com/fullsize/Recombinant-Human-Enolase-1-Protein-Western-Blot-NBP1-30262-img0003.jpg "Western Blot: Recombinant Human Enolase 1 Protein [NBP1-30262] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Enolase 1 antibody [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, MA, PAGE, Bioactivity |
| Concentration | 1 mg/ml |
| Description | An un-tagged recombinant protein corresponding to the amino acids 1-434 of Human Enolase 1 Source: E.coli Amino Acid Sequence: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK |
| Details of Functionality | Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | ENO1 |
| Purity | >95%, by SDS-PAGE |
| Endotoxin Note | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
| Dilutions |
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| Application Notes | Use in Microarray reported in scientific literature (PMID:35059422) |
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| Theoretical MW | 47.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 7.5), 1 mM MgSO4, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE |
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| Gene Symbol | ENO1 |
![SDS-Page: Recombinant Human Enolase 1 Protein [NBP1-30262] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human Enolase 1 Protein-SDS-Page-NBP1-30262-img0004.jpg "SDS-Page: Recombinant Human Enolase 1 Protein [NBP1-30262] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
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