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Endomucin/Mucin -14 Antibody [Unconjugated]

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Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more
MLK2/3-deficient mice exhibit defects in native collateral artery formation.(A) Representative confocal images (n = 5 mice) of control and MLK2/3-deficient whole mount P6 adductor muscle vasculature stained with ...read more
NOX2 contributes to the oxidative stress of bone ECs and the damage of angiogenesis-osteogenesis coupling. (A) qPCR analysis of the relative mRNA levels of three critical NOXs, involved in endothelial oxidative ...read more
The effects of anti-oxidant treatments on the bone blood vessels of T1DM mice. T1D mice received intraperitoneal injection of an anti-oxidant, N-acetyl cysteine (NAC) or tempol (TPO), once every other day from week 4 ...read more
Endothelial JNK-deficient mice display abnormal native collateral arteries.(A,B) Representative confocal images (n = 7 mice) of whole mount adductor muscle vasculature reveals SMA-covered gracilis collateral arteries ...read more
The effects of insulin therapy on the bone blood vessels of T1DM mice. Drug therapy with high dose (hi, 0.5 U/mouse) or low dose (lo, 0.05 U/mouse) of insulin (ins) or the combination of insulin and a relatively low ...read more
VE-cadherin expression is retained in the vasculature of affected organs in CYP19A1(Tg)Cre; Cdh5fl/fl embryos.(A) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl brains for VE-cadherin (green) ...read more
VE-cadherin expression is retained in the vasculature of affected organs in CYP19A1(Tg)Cre; Cdh5fl/fl embryos.(A) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl brains for VE-cadherin (green) ...read more
VE-cadherin expression is retained in the vasculature of affected organs in CYP19A1(Tg)Cre; Cdh5fl/fl embryos.(A) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl brains for VE-cadherin (green) ...read more
VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium.(A) Immunofluorescence staining of E12.5 Control&CYP19A1(Tg)Cre; Cdh5fl/fl placentas for Endomucin (green)&alpha-smooth ...read more
Loss of trophoblast VE-cadherin causes defects in brain, liver, and heart development.(A) Sagittal hematoxylin and eosin (H&E) sections from E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl embryos. Boxes show magnified ...read more
VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium. (G, H) Maximum intensity projections of whole-mount immunofluorescence of the decidua (G)&labyrinth (H) from 200 μm ...read more
Deletion of VE-cadherin in trophoblasts disrupts cell migration resulting in placental&fetal growth restriction. (I–L) Hematoxylin&eosin (H&E) staining&immunofluorescence staining for Endomucin (green), CK8 ...read more
VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium. (G, H) Maximum intensity projections of whole-mount immunofluorescence of the decidua (G)&labyrinth (H) from 200 μm ...read more
Deletion of VE-cadherin in trophoblasts disrupts cell migration resulting in placental&fetal growth restriction. (I–L) Hematoxylin&eosin (H&E) staining&immunofluorescence staining for Endomucin (green), CK8 ...read more
VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium.(D) Immunofluorescence staining of E12.5 Control&CYP19A1(Tg)Cre; Cdh5fl/fl placentas for Endomucin (green)&CK8 (red). ...read more
Disrupting trophoblast migration results in decidual extracellular matrix defects.(A) Mmp15 mRNA gene expression in control and CYP19A1(Tg)Cre; Cdh5fl/fl placentas from RNA-sequencing (RNA-seq). (B) Immunofluorescence ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Endomucin/Mucin -14 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Endomucin
Glu21-Glu90
Accession # Q9R0H2
Specificity
Detects mouse Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Endomucin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EMCN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4666 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endomucin/Mucin -14 Antibody [Unconjugated]

  • EMCN
  • EMCN2
  • Endomucin
  • MUC-14

Background

Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80‑120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21‑190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91‑141, a second shows a one aa substitution for aa 91‑129, and a third shows a one aa substitution for aa 129‑142. Over
aa 21‑90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endomucin/Mucin -14 Antibody (AF4666)(21)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 6 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(8)
IHC-Fr
(3)
IHC-P
(1)
Immunohistochemistry
(2)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(12)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF4666 Applications Species
He, H;Bell, SM;Davis, AK;Zhao, S;Sridharan, A;Na, CL;Guo, M;Xu, Y;Snowball, J;Swarr, DT;Zacharias, WJ;Whitsett, JA; PRDM3/16 regulate chromatin accessibility required for NKX2-1 mediated alveolar epithelial differentiation and function Nature communications 2024-09-16 [PMID: 39284798] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Gao, X;Murphy, MM;Peyer, JG;Ni, Y;Yang, M;Zhang, Y;Guo, J;Kara, N;Embree, C;Tasdogan, A;Ubellacker, JM;Crane, GM;Fang, S;Zhao, Z;Shen, B;Morrison, SJ; Leptin receptor+ cells promote bone marrow innervation and regeneration by synthesizing nerve growth factor Nature cell biology 2023-11-27 [PMID: 38012403] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Niethamer, TK;Levin, LI;Morley, MP;Babu, A;Zhou, S;Morrisey, EE; Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration eLife 2023-05-26 [PMID: 37233732] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Hiroshi YOMOGITA, Hikaru ITO, Kento HASHIMOTO, Akihiko KUDO, Toshiaki FUKUSHIMA, Tsutomu ENDO et al. A possible function of <i>Nik-related kinase</i> in the labyrinth layer of delayed delivery mouse placentas Journal of Reproduction and Development 2/8/2023 [PMID: 36567126]
EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse) IHC Mouse
Prashant Chandrasekaran, Nicholas M. Negretti, Aravind Sivakumar, Derek C. Liberti, Hongbo Wen, Maureen Peers de Nieuwburgh et al. CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth Development 11/1/2022 [PMID: 36239312]
Feng Gao, Changgong Li, Susan M Smith, Neil Peinado, Golenaz Kohbodi, Evelyn Tran et al. Decoding the IGF1 signaling gene regulatory network behind alveologenesis from a mouse model of bronchopulmonary dysplasia eLife 10/10/2022 [PMID: 36214448]
Q Wang, B Zhang, B Stutz, ZW Liu, TL Horvath, X Yang Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity Science Advances, 2022-08-31;8(35):eabn8092. 2022-08-31 [PMID: 36044565] (Western Blot, Mouse) Western Blot Mouse
AC Daquinag, Z Gao, Y Yu, MG Kolonin Endothelial TrkA coordinates vascularization and innervation in thermogenic adipose tissue and can be targeted to control metabolism Molecular Metabolism, 2022-07-11;63(0):101544. 2022-07-11 [PMID: 35835372] (IHC, Mouse) IHC Mouse
David Rohde, Katrien Vandoorne, I-Hsiu Lee, Jana Grune, Shuang Zhang, Cameron S. McAlpine et al. Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease Nature Cardiovascular Research 6/24/2022 [PMID: 35747128]
Show All 21 Publications.

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Bioinformatics

Gene Symbol EMCN
Uniprot