Home » IGFBP-rp1/IGFBP-7 » IGFBP-rp1/IGFBP-7 Antibodies » IGFBP-rp1/IGFBP-7 Antibody [Unconjugated]
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IGFBP-rp1/IGFBP-7 Antibody [Unconjugated]

 (13 Publications)
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP-rp1/IGFBP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP-rp1/IGFBP-7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP-rp1/IGFBP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) ...read more
IGFBP-rp1/IGFBP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP-rp1/IGFBP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1334
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
BAF1334
Carrier Free
AF1334-SP
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IGFBP-rp1/IGFBP-7 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1
Arg98-Arg277
Accession # AAA16187
Specificity
Detects human IGFBP-rp1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IGFBP‑L1, rhIGFBP-rp10 and recombinant mouse IGFBP-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGFBP7
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1334 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-rp1/IGFBP-7 Antibody [Unconjugated]

  • angiomodulin
  • FSTL2
  • IBP-7
  • IGFBP7
  • IGFBP-7
  • IGFBP-7PGI2-stimulating factor
  • IGFBP-7v
  • IGFBPrp1
  • IGFBP-rp1
  • insulin-like growth factor binding protein 7
  • insulin-like growth factor-binding protein 7
  • MAC25 protein
  • Mac25
  • MAC25IGF-binding protein 7
  • Prostacyclin-stimulating factor
  • PSF
  • PSFAGM
  • TAF
  • Tumor-derived adhesion factor

Background

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. Mature IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP-1 through 5. Human and mouse IGFBP-rp1 are highly homologous. Discounting a segment of 43 aa near the N-terminus that is missing in the mouse homologue, human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGFBP-rp1/IGFBP-7 Antibody (AF1334)(13)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 4 applications: ELISA Development, ICC, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC-P
(2)
Western Blot
(6)
All Applications
Filter By Species
Human
(8)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF1334 Applications Species
Yap L, Chong LY, Tan C et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs npj Regenerative Medicine 2023-05-26 [PMID: 37236990]
Yap L, Chong LY, Tan C et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs npj Regenerative Medicine 5/26/2023 [PMID: 37236990]
M Ackermann, JC Kamp, C Werlein, CL Walsh, H Stark, V Prade, R Surabattul, WL Wagner, C Disney, AJ Bodey, T Illig, DJ Leeming, MA Karsdal, A Tzankov, P Boor, MP Kühnel, FP Länger, SE Verleden, HM Kvasnicka, HH Kreipe, A Haverich, SM Black, A Walch, P Tafforeau, PD Lee, MM Hoeper, T Welte, B Seeliger, S David, D Schuppan, SJ Mentzer, DD Jonigk The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling EBioMedicine, 2022-10-04;85(0):104296. 2022-10-04 [PMID: 36206625] (ELISA Development, Human) ELISA Development Human
Lily L. Remsing Remsing Rix, Natalia J. Sumi, Qianqian Hu, Bina Desai, Annamarie T. Bryant, Xueli Li et al. IGF-binding proteins secreted by cancer-associated fibroblasts induce context-dependent drug sensitization of lung cancer cells Science Signaling 8/16/2022 [PMID: 35973030]
A Yogi, M Rukhlova, C Charlebois, G Tian, DB Stanimirov, MJ Moreno Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications Biomedicines, 2021-07-09;9(7):. 2021-07-09 [PMID: 34356861] (ICC, Western Blot, Human, Porcine) ICC, Western Blot Human, Porcine
Shuting Zhu, Hong Wang, Zhihua Zhang, Mingming Ma, Zhi Zheng, Xun Xu et al. IGFBP‑rP1‑silencing promotes hypoxia‑induced angiogenic potential of choroidal endothelial cells via the RAF/MEK/ERK signaling pathway Molecular Medicine Reports 10/11/2020 [PMID: 33173998]
M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell, 2019-09-19;25(4):514-530.e8. 2019-09-19 [PMID: 31543366] (Western Blot, Human) Western Blot Human
Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al. Unique Transcriptional Programs Identify Subtypes of AKI Journal of the American Society of Nephrology 6/1/2017 [PMID: 28028135]
Cheriyan V, Wang Y, Muthu M, Jamal S, Chen D, Yang H, Polin L, Tarca A, Pass H, Dou Q, Sharma S, Wali A, Rishi A Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis. PLoS ONE, 2014-04-01;9(4):e93711. 2014-04-01 [PMID: 24690739] (Western Blot, Human) Western Blot Human
Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction. Oncogene, 2014-03-17;34(7):815-25. 2014-03-17 [PMID: 24632618] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol IGFBP7
Uniprot