![Western Blot: EEF1A2 Antibody [NBP2-33280] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP2-33280-img0013.jpg "Western Blot: EEF1A2 Antibody [NBP2-33280] - Analysis in human cell line RPMI-8226.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: LLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGIL |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EEF1A2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP2-33280 | Applications | Species |
|---|---|---|
| Qiu FN, Huang Y, Chen DY et al. Eukaryotic elongation factor-1alpha 2 knockdown inhibits hepatocarcinogenesis by suppressing PI3K/Akt/NF-kB signaling. World J. Gastroenterol. 2016-04-28 [PMID: 27122673] (IHC-P, WB, Human) | IHC-P, WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for EEF1A2 Antibody (NBP2-33280)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: EEF1A2 Antibody [NBP2-33280] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33280-img0007.jpg "Immunocytochemistry/Immunofluorescence: EEF1A2 Antibody [NBP2-33280] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human skeletal muscle shows weak cytoplasmic positivity in a subset of myocytes.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-33280-img0012.jpg "Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human skeletal muscle shows weak cytoplasmic positivity in a subset of myocytes.")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-33280-img0009.jpg "Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-33280-img0010.jpg "Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-33280-img0011.jpg "Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")