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MCF2L Antibody (1D11)

Images

 
Immunohistochemistry-Paraffin: MCF2L Antibody (1D11) [H00023263-M01] - Analysis of monoclonal antibody to MCF2L on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Sandwich ELISA: MCF2L Antibody (1D11) [H00023263-M01] - Detection limit for recombinant GST tagged MCF2L is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
1D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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MCF2L Antibody (1D11) Summary

Immunogen
MCF2L (AAH20208, 220 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCL
Specificity
MCF2L - MCF.2 cell line derived transforming sequence-like
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCF2L
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCF2L Antibody (1D11)

  • DBL's big sister
  • DBS
  • FLJ12122
  • guanine nucleotide exchange factor DBS
  • KIAA0362ARHGEF14
  • MCF.2 cell line derived transforming sequence-like
  • MCF2 transforming sequence-like protein
  • MCF2-transforming sequence-like protein
  • OST

Background

Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus . Isoform 5 activates CDC42

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array H00023263-M01

Research Areas for MCF2L Antibody (H00023263-M01)

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Bioinformatics

Gene Symbol MCF2L
Entrez
Uniprot