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DIAPH-2 Antibody (CL1111)

Images

 
Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human placenta shows strong staining in the trophoblast.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clone
CL1111
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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DIAPH-2 Antibody (CL1111) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQIKTLADDVRDRITSFRKSTVKKEKPLIQHPIDSQVAMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDMNLNEEKKAPLRNKDFTTKREMVVQYISAT
Epitope
SLNLSEKEVLDLFEK
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DIAPH2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DIAPH-2 Recombinant Protein Antigen (NBP2-52966PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for DIAPH-2 Antibody (CL1111)

  • DIA2
  • DIAdiaphanous (Drosophila, homolog) 2
  • DIAPH2
  • DIAPH-2
  • diaphanous homolog 2 (Drosophila)
  • Diaphanous-related formin-2
  • diaphorase-2
  • DRF2
  • POF
  • POF2
  • POF2FLJ11167
  • protein diaphanous homolog 2

Background

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DIAPH2