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DIAPH-2 Antibody (699142)

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Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIAPH-2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
699142
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6806-SP
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DIAPH-2 Antibody (699142) Summary

Specificity
Detects human DIAPH-2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DIAPH2
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DIAPH-2 Antibody (699142)

  • DIA2
  • DIAdiaphanous (Drosophila, homolog) 2
  • DIAPH2
  • DIAPH-2
  • diaphanous homolog 2 (Drosophila)
  • Diaphanous-related formin-2
  • diaphorase-2
  • DRF2
  • POF
  • POF2
  • POF2FLJ11167
  • protein diaphanous homolog 2

Background

DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DIAPH-2 Antibody
(699142)
MAB6806
DIAPH-2 Antibody (699142)
Species: Hu
Applications: WB

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DIAPH2
Uniprot