![Western Blot: DCK Antibody (OTI3F5) [NBP2-00590] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).](http://images.novusbio.com/fullsize/DCK-Antibody-OTI3F5-Western-Blot-NBP2-00590-img0015.jpg "Western Blot: DCK Antibody (OTI3F5) [NBP2-00590] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DCK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 30.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: DCK Antibody (OTI3F5) [NBP2-00590] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK.](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Immunocytochemistry-Immunofluorescence-NBP2-00590-img0011.jpg "Immunocytochemistry/Immunofluorescence: DCK Antibody (OTI3F5) [NBP2-00590] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK.")
![Immunohistochemistry-Paraffin: DCK Antibody (3F5) [NBP2-00590] - Staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-00590-img0013.jpg "Immunohistochemistry-Paraffin: DCK Antibody (3F5) [NBP2-00590] - Staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody.")
![Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCK antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Flow-Cytometry-NBP2-00590-img0010.jpg "Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCK antibody, and then analyzed by flow cytometry.")
![Western Blot: DCK Antibody (3F5) [NBP2-00590] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Western-Blot-NBP2-00590-img0014.jpg "Western Blot: DCK Antibody (3F5) [NBP2-00590] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.")
![Immunohistochemistry-Paraffin: DCK Antibody (3F5) [NBP2-00590] - Staining of paraffin-embedded Human lymph node tissue using anti-DCK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-00590-img0012.jpg "Immunohistochemistry-Paraffin: DCK Antibody (3F5) [NBP2-00590] - Staining of paraffin-embedded Human lymph node tissue using anti-DCK mouse monoclonal antibody.")
![Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - Analysis of Hela cells, using anti-DCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Flow-Cytometry-NBP2-00590-img0008.jpg "Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - Analysis of Hela cells, using anti-DCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - Analysis of Jurkat cells, using anti-DCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCK-Antibody-3F5-Flow-Cytometry-NBP2-00590-img0009.jpg "Flow Cytometry: DCK Antibody (3F5) [NBP2-00590] - Analysis of Jurkat cells, using anti-DCK antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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