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DGK-beta Antibody (646523)

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Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
646523
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6727-SP
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DGK-beta Antibody (646523) Summary

Specificity
Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha , gamma , epsilon , iota , kappa , theta , or zeta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DGKB
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-beta Antibody (646523)

  • 90 kDa diacylglycerol kinase
  • DAG kinase beta
  • DAGK2
  • DGKB
  • DGKbeta
  • DGK-beta
  • diacylglycerol kinase beta
  • diacylglycerol kinase, beta (90kD)
  • diacylglycerol kinase, beta 90kDa
  • Diglyceride kinase beta
  • EC 2.7.1
  • EC 2.7.1.107
  • KIAA0718DGKDGK-BETA

Background

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol DGKB
Uniprot