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Deoxycytidylate deaminase Antibody (4B9)

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Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD expression in transfected 293T cell line by DCTD monoclonal antibody (M01), clone 4B9.Lane 1: DCTD transfected lysate(20.016 ...read more
Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of monoclonal antibody to DCTD on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Genetic Strategies: Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi ( Cat # H00001635-R01V ) ...read more
Sandwich ELISA: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Detection limit for recombinant GST tagged DCTD is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, KD
Clone
4B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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Deoxycytidylate deaminase Antibody (4B9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
DCTD (NP_001912.2, 69 a.a. ~ 178 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RTAENKLDTKYPYVCHAELNAIMNKNSTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSDKYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQKLQ
Specificity
DCTD - dCMP deaminase
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DCTD
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, ELISA and RNAi Validation.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Deoxycytidylate deaminase Antibody (4B9)

  • dCMP deaminaseEC 3.5.4.12
  • deoxycytidylate deaminase
  • MGC111062

Background

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DCTD
Entrez
OMIM
Uniprot