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ADK Antibody

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Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse liver. ADK stained by Adenosine kinase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ADK Antibody [NBP2-15291] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADK antibody (NBP2-15291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.ADK stained by ADK antibody (NBP2-15291) diluted at ...read more
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.ADK stained by ADK antibody (NBP2-15291) diluted at ...read more
Genetic Strategies: Western Blot: ADK Antibody [NBP2-15291] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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ADK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ADK. The exact sequence is proprietary.
Predicted Species
Bovine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADK
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-15291 in the following applications:

Publications
Read Publication using NBP2-15291.

Reactivity Notes

Chicken (83%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for ADK Antibody

  • adenosine kinase
  • ADK
  • AK
  • AKAdenosine 5'-phosphotransferase
  • EC 2.7.1
  • EC 2.7.1.20

Background

This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, RM
Applications: WB, ICC/IF, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ADK Antibody (NBP2-15291)(1)

We have publications tested in 1 application: WB.


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Review for ADK Antibody (NBP2-15291) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP2-15291:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Chiafeng Kuo
WB Other 06/13/2015
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Summary

ApplicationWestern Blot
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ADK Antibody (NBP2-15291) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ADK Products

Research Areas for ADK Antibody (NBP2-15291)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Chiafeng Kuo
06/13/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol ADK
Uniprot