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![Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP2-15291-img0016.jpg "Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15291-img0005.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse liver. ADK stained by Adenosine kinase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunohistochemistry-Paraffin-NBP2-15291-img0014.jpg)
![Western Blot: ADK Antibody [NBP2-15291] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADK antibody (NBP2-15291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024202191.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024205663.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-91020242024612.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ADK Antibody [NBP2-15291] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-161020241940573.jpg)
![Western Blot: ADK Antibody [NBP1-90197] - Analysis in human cell lines PC-3 and A-549 using anti-ADK antibody. Corresponding ADK RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-90197-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ADK Antibody [NBP1-90197] - Analysis in human cell lines PC-3 and A-549 using anti-ADK antibody. Corresponding ADK RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP1-90197] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90197-img0009.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP1-90197] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunohistochemistry-Paraffin-NBP1-90197-img0012.jpg)
![Western Blot: ADK Antibody [NBP1-90197] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-90197-img0008.jpg)
![Western Blot: ADK Antibody [NBP1-31802] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-31802-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ADK Antibody [NBP1-31802] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP1-31802] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31802-img0004.jpg)
![Western Blot: ADK Antibody [NBP1-31802] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP1-31802-img0003.jpg)
![Western Blot: ADK Antibody (4F8) [NBP1-97934] - The cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ADK-Antibody-4F8-Western-Blot-NBP1-97934-img0001.jpg "Western Blot: ADK Antibody (4F8) [NBP1-97934] - The cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: ADK Antibody (4F8) [NBP1-97934] - Analysis in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ADK-Antibody-4F8-Immunocytochemistry-Immunofluorescence-NBP1-97934-img0002.jpg)
![Flow Cytometry: ADK Antibody (4F8) [NBP1-97934] - Flow cytometry analysis of ADK in A549 cell line, staining at 2- 5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/ADK-Antibody-4F8-Flow-Cytometry-NBP1-97934-img0003.jpg)
![Immunoprecipitation: ADK Antibody [NBP2-32189] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Adenosine Kinase antibody NBP2-32189 used for IP at 6 ug per reaction. Adenosine Kinase was also immunoprecipitated by rabbit anti-Adenosine Kinase antibody BL15848. For blotting immunoprecipitated Adenosine Kinase, NBP2-32189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunoprecipitation-NBP2-32189-img0003.jpg "Immunoprecipitation: ADK Antibody [NBP2-32189] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Adenosine Kinase antibody NBP2-32189 used for IP at 6 ug per reaction. Adenosine Kinase was also immunoprecipitated by rabbit anti-Adenosine Kinase antibody BL15848. For blotting immunoprecipitated Adenosine Kinase, NBP2-32189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ADK Antibody [NBP2-32189] - Wholecell lysate (50 ug) from HEK293T, LNCaP, and MCF-7 cellsprepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-ADK antibody usedfor WB at 0.4 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp2-32189_rabbit-polyclonal-adk-antibody-western-blot-2922024103050..jpg)
![Western Blot: ADK Antibody [NBP2-32189] - : Wholecell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-ADK antibody used for WB at 0.4 ug/ml. Detection:Chemiluminescence with an exposure time of 10 seconds](http://images.novusbio.com/fullsize/antibody/nbp2-32189_rabbit-polyclonal-adk-antibody-western-blot-292202410329..jpg)
![Western Blot: ADK Antibody (S05-0G0) [NBP3-19831] - Western blot detection of ADK in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19831 (1:1000 diluted). Predicted band size: 41kDa. Observed band size: 41kDa.](http://images.novusbio.com/fullsize/ADK-Antibody-S05-0G0-Western-Blot-NBP3-19831-img0002.jpg "Western Blot: ADK Antibody (S05-0G0) [NBP3-19831] - Western blot detection of ADK in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19831 (1:1000 diluted). Predicted band size: 41kDa. Observed band size: 41kDa.")
![Immunohistochemistry-Paraffin: ADK Antibody (S05-0G0) [NBP3-19831] - Immunohistochemistry of ADK in paraffin-embedded Human tonsil using NBP3-19831 at dilution 1/50](http://images.novusbio.com/fullsize/ADK-Antibody-S05-0G0-Immunohistochemistry-Paraffin-NBP3-19831-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21383_rabbit-adk-pab-4520231553133.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
![Immunohistochemistry: ADK Antibody [NBP2-38410] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunohistochemistry-NBP2-38410-img0001.jpg "Immunohistochemistry: ADK Antibody [NBP2-38410] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.")
![Western Blot: ADK Overexpression Lysate [NBP2-10955] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenosine Kinase.](http://images.novusbio.com/fullsize/Adenosine-Kinase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10955-img0001.jpg "Western Blot: ADK Overexpression Lysate [NBP2-10955] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenosine Kinase.")
![Western Blot: ADK Antibody [NBP3-00077] - Anti-ADK rabbit polyclonal antibody at 1:500 dilution. Lane A: RAW264.7 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: NIH/3T3 Whole Cell Lysate. Lane D: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP3-00077-img0002.jpg "Western Blot: ADK Antibody [NBP3-00077] - Anti-ADK rabbit polyclonal antibody at 1:500 dilution. Lane A: RAW264.7 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: NIH/3T3 Whole Cell Lysate. Lane D: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).")
![ELISA: Human ADK ELISA Kit (Colorimetric) [NBP3-27183] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27183_human-adk-elisa-kit-colorimetric-11420241025364.png "ELISA: Human ADK ELISA Kit (Colorimetric) [NBP3-27183] - Standard Curve Reference")