Orthogonal Strategies: Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining in human fallopian tube and small intestine tissues using anti-AK1 antibody. Corresponding AK1 RNA-seq ...read more
Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human small intestine shows low expression as expected.
Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human fallopian tube shows high expression.
This antibody was developed against Recombinant Protein corresponding to amino acids: EKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AK1
Purity
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rhesus macaque reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Adenylate Kinase 1 Antibody
Adenylate Kinase 1
adenylate kinase isoenzyme 1
AK 1
AK1
ATP-AMP transphosphorylase 1
EC 2.7.4
EC 2.7.4.3
EC 2.7.4.6
Myokinase
Background
Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Adenylate Kinase 1 Antibody (NBP1-87401) (0)
There are no publications for Adenylate Kinase 1 Antibody (NBP1-87401).
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Review for Adenylate Kinase 1 Antibody (NBP1-87401) (1)
51
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Rhesus Macaque.
Reviews using NBP1-87401:
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Images
Ratings
Applications
Species
Date
Details
5
reviewed by: Addie Luo
WB
Rhesus Macaque
08/13/2021
View
Summary
Application
Western Blot
Sample Tested
Adult lung
Species
Rhesus Macaque
Lot
A06481
Comments
Comments
***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
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