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AK3L1 Antibody (OTI3B1)

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Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] Analysis of extracts (35ug) from 9 different cell lines by using anti-AK3L1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK3L1.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human liver tissue using anti-AK3L1 mouse monoclonal antibody.
Flow Cytometry: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3L1 antibody, and then analyzed ...read more
Western Blot: AK3L1 Antibody (3B1) [NBP2-00827] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human colon tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human endometrium tissue using anti-AK3L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AK3L1 Antibody (3B1) [NBP2-00827] - Staining of paraffin-embedded Human Kidney tissue using anti-AK3L1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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AK3L1 Antibody (OTI3B1) Summary

Immunogen
Full length human recombinant protein of human AK4(NP_037542) produced in HEK293 cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AK3L1 Antibody (OTI3B1)

  • adenylate kinase 3
  • adenylate kinase 3-like 1
  • Adenylate kinase 3-like
  • adenylate kinase 4
  • AK 4
  • AK3
  • AK3adenylate kinase isoenzyme 4, mitochondrial
  • AK3L1
  • AK3L2
  • AK4
  • ATP-AMP transphosphorylase
  • EC 2.7.4
  • EC 2.7.4.3
  • GTP:AMP phosphotransferase
  • MGC166959
  • mitochondrial adenylate kinase-3
  • nucleoside-triphosphate-adenylate kinase

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AK4
Entrez