![Western Blot: AK5 Antibody (2F9) [NBP2-02978] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Western-Blot-NBP2-02978-img0004.jpg "Western Blot: AK5 Antibody (2F9) [NBP2-02978] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2F9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.86 mg/ml |
| Immunogen | Full length human recombinant protein of human AK5 (NP_777283) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AK5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 63.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.86 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot Syntaxin-BP3/Munc18c Antibody [Unconjugated]](https://images.novusbio.com/images2/Syntaxin-BP3_AF5659_Western_Blot_12110.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: AK5 Antibody (2F9) [NBP2-02978] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunocytochemistry-Immunofluorescence-NBP2-02978-img0022.jpg "Immunocytochemistry/Immunofluorescence: AK5 Antibody (2F9) [NBP2-02978] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human prostate tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0021.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human prostate tissue using anti-AK5 mouse monoclonal antibody.")
![Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Flow-Cytometry-NBP2-02978-img0003.jpg "Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0005.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0006.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0007.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0008.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0009.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0010.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0011.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0012.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0013.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human bladder tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0014.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human bladder tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human breast tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0015.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human breast tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human colon tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0016.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human colon tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human Kidney tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0017.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human Kidney tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human liver tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0018.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human liver tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human lung tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0019.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human lung tissue using anti-AK5 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human pancreas tissue using anti-AK5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Immunohistochemistry-Paraffin-NBP2-02978-img0020.jpg "Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human pancreas tissue using anti-AK5 mouse monoclonal antibody.")
![Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - Analysis of Hela cells, using anti-AK5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Flow-Cytometry-NBP2-02978-img0001.jpg "Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - Analysis of Hela cells, using anti-AK5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - Analysis of Jurkat cells, using anti-AK5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AK5-Antibody-2F9-Flow-Cytometry-NBP2-02978-img0002.jpg "Flow Cytometry: AK5 Antibody (2F9) [NBP2-02978] - Analysis of Jurkat cells, using anti-AK5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0004.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
