![Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Human breast cancer MCF7 cells transfected with Aldolase C guide RNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Western-Blot-NBP2-25145-img0006.jpg "Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Human breast cancer MCF7 cells transfected with Aldolase C guide RNA. Western blot image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 4A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | The N-terminal 20 amino acids of human Aldolase C (MPHSYPALSAEQKKELSDIA). [UniProt# P09972] |
| Localization | Gray matter astrocytes and cells of the pia mater |
| Marker | pan-Astrocyte Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ALDOC |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Aldolase C (1A1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~40 kDa. |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 10/22/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Aldolase C Antibody (NBP2-25145)Find related products by research area.
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RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - A. Immunofluorescence of rat cerebellum section stained with mouse mAb to aldolase C, NBP2-25145, dilution 1:1000, in green, and costained with rabbit pAb to GFAP, dilution 1:5000 in red. In both A and B blue is Hoechst staining of nuclear DNA. Aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while GFAP antibody stains processes of Bergman glia and astrocytic cells. B. Immunofluorescence of E20 rat cortical culture stained with NBP2-25145, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:10,000 in red. The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Immunocytochemistry-Immunofluorescence-NBP2-25145-img0004.jpg "Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - A. Immunofluorescence of rat cerebellum section stained with mouse mAb to aldolase C, NBP2-25145, dilution 1:1000, in green, and costained with rabbit pAb to GFAP, dilution 1:5000 in red. In both A and B blue is Hoechst staining of nuclear DNA. Aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while GFAP antibody stains processes of Bergman glia and astrocytic cells. B. Immunofluorescence of E20 rat cortical culture stained with NBP2-25145, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:10,000 in red. The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons.")
![Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Analysis of tissue and lysates using mouse mAb to aldolase C, NBP2-25145, dilution 1:2000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus. The single band at about 40 kDa corresponds to the aldolase C protein.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Western-Blot-NBP2-25145-img0005.jpg "Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Analysis of tissue and lysates using mouse mAb to aldolase C, NBP2-25145, dilution 1:2000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus. The single band at about 40 kDa corresponds to the aldolase C protein.")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - View of mixed neuron/glial cultures stained with NBP2-25145 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25145 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Immunocytochemistry-Immunofluorescence-NBP2-25145-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - View of mixed neuron/glial cultures stained with NBP2-25145 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25145 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
