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ACADS Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Analysis in human duodenum and tonsil tissues. Corresponding ACADS RNA-seq data are presented for the same tissues.
Western Blot: ACADS Antibody [NBP1-89290] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: ACADS Antibody [NBP1-89290] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Western Blot: ACADS Antibody [NBP1-89290] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: ...read more
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human tonsil shows granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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ACADS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACADS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
ACADS Protein (NBP1-89290PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACADS Antibody

  • ACAD3
  • acyl-CoA dehydrogenase, C-2 to C-3 short chain
  • acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
  • Butyryl-CoA dehydrogenase
  • EC 1.3.99
  • EC 1.3.99.2
  • mitochondrial short-chain specific acyl-CoA dehydrogenase
  • SCADshort-chain acyl-CoA dehydrogenase
  • short-chain specific acyl-CoA dehydrogenase, mitochondrial
  • unsaturated acyl-CoA reductase

Background

ACADS encodes a a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with Short Chain Acyl-CoA Dehydrogenase Deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACADS