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FANCD2 Antibody - BSA Free

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Biological Strategies: Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Effects of FANCD2 knockdown on levels of cell cycle regulators. Caki-1 cells were transfected ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ca, Ma, Pm, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IP, KD, WB, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Protocols & FAQs
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FANCD2 Antibody - BSA Free Summary

Immunogen
This FANCD2 Antibody was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FANCD2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) reported in scientific literature (PMID 28196964)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 28196964)
  • Flow Cytometry 2 - 5 ug/ml
  • Immunoblotting reported in multiple pieces of scientific literature
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5-5.0 ug/ml
  • Immunohistochemistry-Paraffin 2.5-5.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated
  • RNA Inhibition reported in scientific literature (PMID 27694619)
  • Simple Western 1:25
  • Western Blot 1 - 2 ug/ml
Application Notes
By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence, it has been tested in human MMC and IR treated MEF cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB100-182 in the following applications:

Publications
Read Publications using
NB100-182 in the following applications:

Reactivity Notes

Primate reactivity reported in literature (PMID: 21421661). Canine reactivity reported in literature (PMID: 27257868). Zebrafish reactivity reported in scientific literature (PMID: 30540754). Rat reactivity reported in multiple pieces of scientific literature. Kangaroo reactivity reported in scientific literature (PMID: 24982423).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCD2 Antibody - BSA Free

  • DKFZp762A223
  • FA4
  • FACD
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia complementation group D2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • FLJ23826
  • FPN1
  • HFE4
  • IREG1
  • Protein FACD2
  • SLC11A3

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

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Verified Customer
01/21/2020
Application: IF experiment
Species: Mouse

Verified Customer
01/22/2018
Application: WB
Species: humam cell lines

Lisa Moreau
12/15/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol FANCD2