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ACADL Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Analysis in human thyroid gland and cerebral cortex tissues. Corresponding ACADL RNA-seq data are presented for the same tissues.
Western Blot: ACADL Antibody [NBP1-89289] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human lung shows moderate to strong granular cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human cerebral cortex shows no positivity as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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ACADL Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GEERLETPSAKKLTDIGIRRIFSPEHDIFRKSVRKFFQEEVIPHHSEWEKAGEVSREVWEKAGKQGLLGVNIAEHLGGIGGDLYSAAIVWEEQAYSNCSGPGFSIHSGIVMSYITNHGSEEQIKHFIPQMT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACADL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ACADL Protein (NBP1-89289PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACADL Antibody

  • ACAD4
  • acyl-CoA dehydrogenase, long chain
  • acyl-Coenzyme A dehydrogenase, long chain
  • EC 1.3.99
  • EC 1.3.99.13
  • LCADFLJ94052
  • long-chain specific acyl-CoA dehydrogenase, mitochondrial

Background

The protein encoded by the ACADL gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrialflavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymesthat catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene arethe cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional ACADL Products

Array NBP1-89289

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Bioinformatics

Gene Symbol ACADL