Aconitase 1 Antibody

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Immunohistochemistry, WB.

Showing Publications 1 - 3 of 3.

Publications using NBP1-87677	Applications	Species
Gu W, Fillebeen C, Pantopoulos K. Human IRP1 Translocates to the Nucleus in a Cell-Specific and Iron-Dependent Manner International Journal of Molecular Sciences 2022-09-15 [PMID: 36142654] (Immunohistochemistry)	Immunohistochemistry
Dongiovanni P, Lanti C, Gatti S et al. High fat diet subverts hepatocellular iron uptake determining dysmetabolic iron overload PLoS ONE. 2015-02-04 [PMID: 25647178] (WB, Human, Rat) Details: Aconitase 1 / IRP1 antibody used for WB on lysates of liver tissues from Rats which were fed regular diet, high-fat diet/HFD or HFD supplemented with iron/HFD + Iron (Figure 4C). WB also performed on lysates of human HepG2 hepatocytes which were treated or not with palmitic acid/PA (0.66 mM: saturated, C16:0) and oleic acid/OA (1 mM: n-9 mono-unsaturated, C18:1, cis), PA + OA + ferric ammonium citrate (FAC, 150 uM) or FAC only (Figure 6A).	WB	Human, Rat
Haro KJ, Sheth A, Scheinberg DA et al. Dysregulation of IRP1-Mediated Iron Metabolism Causes Gamma Ray-specific Radioresistance in Leukemia Cells. PLoS One 2012-01-01 [PMID: 23155415]

Images	Ratings	Applications	Species	Date	Details
Enlarge	1 reviewed by: Nicholas Niemuth	WB	Bovine and Chironomus riprius	03/09/2020	View Summary Application Western Blot Sample Tested Purified protein,Whole organism protein extract Species Bovine and Chironomus riprius Lot A105066 Comments Comments *Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
	4 reviewed by: Verified Customer	WB	Mouse	07/28/2014	View Summary Application Western Blot Sample Tested mouse lung Species Mouse

Array NBP1-87677

• Aconitase 1 Antibodies
• Aconitase 1 ELISA Kit
• Aconitase 1 Lysate
• Aconitase 1 Proteins