IRP2 Antibody

Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: GS, IHC, WB.

Showing Publications 1 - 10 of 10.

Publications using NB100-1798	Applications	Species
Zhuanzhuan Liu, Hanying Wang, Zhiwei Zhang, Yulu Ma, Qiyue Jing, Shenghai Zhang, Jinzhi Han, Junru Chen, Yaoyao Xiang, Yanbo Kou, Yanxia Wei, Lu Wang, Yugang Wang, Igor C. Almeida Fam96a is essential for the host control of Toxoplasma gondii infection by fine-tuning macrophage polarization via an iron-dependent mechanism PLOS Neglected Tropical Diseases 2024-05-07 [PMID: 38713713]
Ito F, Kato K, Yanatori I Et al. Ferroptosis-dependent extracellular vesicles from macrophage contribute to asbestos-induced mesothelial carcinogenesis through loading ferritin Redox biology 2021-10-21 [PMID: 34700146] (Human)		Human
Cheng Z, Akatsuka S, Li GH et al. Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis Cancer Science 2022-01-01 [PMID: 34699654] (WB, IHC)	WB, IHC
Jiang Z, Wang Z, Chen L et al. Artesunate induces ER-derived-ROS-mediated cell death by disrupting labile iron pool and iron redistribution in hepatocellular carcinoma cells American journal of cancer research 2021-03-01 [PMID: 33791148] (WB, Human)	WB	Human
Masaldan S, Clatworthy SAS, Gamell C et al. Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis Redox Biol 2017-09-01 [PMID: 28888202] (WB, Human)	WB	Human
Cloonan SM, Glass K, Laucho-Contreras ME et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 2016-02-01 [PMID: 26752519] (WB, GS, Mouse)	WB, GS	Mouse
Whitnall M, Rahmanto YS, Huang ML et al. Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia Proc Natl Acad Sci U S A 2012-11-20 [PMID: 23169664] (WB, Mouse)	WB	Mouse
Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K. Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency. Free Radic Res. 2012-04-10 [PMID: 22435664] (WB, Mouse)	WB	Mouse
Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT. Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet. Eur J Nutr. 2012-01-13 [PMID: 22241739] (WB, Mouse)	WB	Mouse
Regan RF, Chen M, Li Z et al. Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin. Neurobiol Dis 2008-08-01 [PMID: 18571425] (Mouse)		Mouse

Array NB100-1798

• IRP2 Antibodies
• IRP2 Lysate
• IRP2 Proteins