![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 6D3A10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 3/CHRNA3 (AA: 32-240) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHRNA3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NBP2-61673 | Applications | Species |
|---|---|---|
| Wei N, Chu Y, Liu H Et al. Antagonistic Mechanism of alpha-Conotoxin BuIA toward the Human alpha3beta2 Nicotinic Acetylcholine Receptor ACS chemical neuroscience 2021-11-05 [PMID: 34738810] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (NBP2-61673)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CHRNA3 |
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0007.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).")
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0006.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0002.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).")
![ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-ELISA-NBP2-61673-img0001.jpg "ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![Hip Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Hip Antibody [Unconjugated]")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")