![Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Western-Blot-NBP2-61679-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 5B6G8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 6/CHRNA6 (AA: 26-239) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHRNA6 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (NBP2-61679)Find related products by research area.
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| Gene Symbol | CHRNA6 |
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Immunocytochemistry-Immunofluorescence-NBP2-61679-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Immunohistochemistry-NBP2-61679-img0004.jpg "Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Flow-Cytometry-NBP2-61679-img0007.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).")
![Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Western-Blot-NBP2-61679-img0006.jpg "Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Flow-Cytometry-NBP2-61679-img0002.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).")
![ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-ELISA-NBP2-61679-img0001.jpg "ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")