![Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against human CHRNA7 (AA: extra 52-259) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-Western-Blot-NBP2-61739-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against human CHRNA7 (AA: extra 52-259) recombinant protein. (Expected MW is 50.4 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 7F10G1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 7/CHRNA7 (AA: extra 52-259) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHRNA7 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 56.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publications using NBP2-61739 | Applications | Species |
|---|---|---|
| Kuol N, Godlewski J, Kmiec Z Et Al. Cholinergic signalling influences the expression of immune checkpoint inhibitors, PD-L1 and PD-L2, in human colorectal cancer tissues and cell lines Research Square 2021-07-07 (ICC/IF) | ICC/IF | |
| Kuol N, Godlewski J, Kmiec Z et al. Cholinergic signaling influences the expression of immune checkpoint inhibitors, PD-L1 and PD-L2, and tumor hallmarks in human colorectal cancer tissues and cell lines BMC cancer 2023-10-12 [PMID: 37828429] (ICC/IF, IHC-P, Human) | ICC/IF, IHC-P | Human |
| Kuol N, Davidson M, Karakkat J et al. Blocking Muscarinic Receptor 3 Attenuates Tumor Growth and Decreases Immunosuppressive and Cholinergic Markers in an Orthotopic Mouse Model of Colorectal Cancer International Journal of Molecular Sciences 2022-12-29 [PMID: 36614038] (IHC-Fr, Mouse) | IHC-Fr | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP2-61739)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CHRNA7 |
![Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of rectum cancer tissues using CHRNA7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-Immunohistochemistry-NBP2-61739-img0004.jpg "Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of rectum cancer tissues using CHRNA7 mouse mAb with DAB staining.")
![Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of SH-SY5Y cells using CHRNA7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-Flow-Cytometry-NBP2-61739-img0002.jpg "Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of SH-SY5Y cells using CHRNA7 mouse mAb (green) and negative control (red).")
![Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against HEK293 (1) and CHRNA7 (AA: extra 52-259)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-Western-Blot-NBP2-61739-img0006.jpg "Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against HEK293 (1) and CHRNA7 (AA: extra 52-259)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of bladder cancer tissues using CHRNA7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-Immunohistochemistry-NBP2-61739-img0003.jpg "Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of bladder cancer tissues using CHRNA7 mouse mAb with DAB staining.")
![ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-7-CHRNA7-Antibody-7F10G1-ELISA-NBP2-61739-img0001.jpg "ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")