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![Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] -Analysis in human kidney and skeletal muscle tissues using NBP1-87677 antibody. Corresponding ACO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87677_rabbit-polyclonal-aconitase-1-antibody-immunohistochemistry-paraffin-19420248441..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] -Analysis in human kidney and skeletal muscle tissues using NBP1-87677 antibody. Corresponding ACO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aconitase 1 Antibody [NBP1-87677] - Analysis in human cell lines U2OS and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP1-87677-img0014.jpg)
![Western Blot: Aconitase 1 Antibody [NBP1-87677] - Effect of HFD on IRP1. Densitometric analysis of IRP1 protein levels; beta-actin is shown as the loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116855), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP1-87677-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody [NBP1-87677] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87677-img0012.jpg)
![Western Blot: Aconitase 1 Antibody [NBP1-87677] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP1-87677-img0010.jpg)
![Western Blot: Aconitase 1 Antibody [NBP1-87677] - Effect of fatty acids on IRP1 expression in HepG2 hepatocytes. IRP1 protein levels were evaluated by Western Blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116855), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP1-87677-img0024.jpg)
![Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87677-img0020.jpg)
![Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP1-87677] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-87677_rabbit-polyclonal-aconitase-1-antibody-immunohistochemistry-paraffin-19420248540..jpg)
![Western Blot: Aconitase 1 Antibody [NBP1-87677] - Effect of HFD on IRP1.A) Total IRP1 activity was measured by RNA band shift assay. B) Densitometric analysis of IRP1 activity. C) Hepatic IRP1 protein levels evaluated by Western Blotting. C) Densitometric analysis of IRP1 protein levels; beta -actin is shown as the loading control. D). Effect of HFD on IRP1 & IRP2 mRNA levels. Gene expression was evaluated by qRT-PCR. The figure is representative of results obtained in 6 animals per group in two independent experiments. Values are expressed as means±SD. AU, arbitrary units. *p<0.05 vs controls. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116855), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87677_rabbit-polyclonal-aconitase-1-antibody-310202415371962.jpg)
![Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] - Positive Western Blot detected in: Hela whole cell lysate. All lanes: Aconitase 1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100 kDa Observed band size: 95-130 kDa](http://images.novusbio.com/fullsize/nbp3-26529_rabbit-aconitase-1-mab-22c10-282202411413617.jpg "Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] - Positive Western Blot detected in: Hela whole cell lysate. All lanes: Aconitase 1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100 kDa Observed band size: 95-130 kDa")
![Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] - Staining of HepG2 cell with Aconitase 1 Antibody (22C10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 511-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26529_rabbit-aconitase-1-mab-22c10-282202412493643.jpg)
![Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] - Overlay Peak curve showing HepG2 cells stained with Aconitase 1 Antibody (22C10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26529_rabbit-aconitase-1-mab-22c10-282202414183636.jpg)
![Immunoprecipitation: Aconitase 1 Antibody (2C1) [H00000048-M01] - Analysis of ACO1 transfected lysate using anti-ACO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-2C1-Immunoprecipitation-H00000048-M01-img0002.jpg "Immunoprecipitation: Aconitase 1 Antibody (2C1) [H00000048-M01] - Analysis of ACO1 transfected lysate using anti-ACO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACO1 MaxPab rabbit polyclonal antibody.")
![ELISA: Aconitase 1 Antibody (2C1) [H00000048-M01] - Detection limit for recombinant GST tagged ACO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-2C1-ELISA-H00000048-M01-img0003.jpg)
![Western Blot: Aconitase 1 Antibody [NBP2-15244] - A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP2-15244-img0003.jpg "Western Blot: Aconitase 1 Antibody [NBP2-15244] - A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody [NBP2-15244] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Aconitase 1 protein stained by Aconitase 1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15244-img0004.jpg)
![Western Blot: Aconitase 1 Antibody (SR2310) [NBP3-22405] - Western blot analysis using NBP3-22405 in (1) mouse kidney lysate; (2) HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22405_rabbit-aconitase-1-mab-sr2310-57202317215192.jpg "Western Blot: Aconitase 1 Antibody (SR2310) [NBP3-22405] - Western blot analysis using NBP3-22405 in (1) mouse kidney lysate; (2) HepG2 cell lysate.")
![Flow Cytometry: Aconitase 1 Antibody (JE63-98) [NBP3-31993] - Flow cytometric analysis of HeLa cells labeling Aconitase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31993, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31993_rabbit-aconitase-1-mab-je63-98-29202416453254.jpg "Flow Cytometry: Aconitase 1 Antibody (JE63-98) [NBP3-31993] - Flow cytometric analysis of HeLa cells labeling Aconitase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31993, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Aconitase 1 Antibody (JE63-98) [NBP3-31993] - Western blot analysis of Aconitase 1 on different lysates with Rabbit anti-Aconitase 1 antibody (NBP3-31993) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HCT 116 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31993_rabbit-aconitase-1-mab-je63-98-3920248485011.jpg)
![Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP3-10536] - Immunohistochemical analysis of paraffin-embedded human muscle tissue.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-10536-img0001.jpg "Immunohistochemistry-Paraffin: Aconitase 1 Antibody [NBP3-10536] - Immunohistochemical analysis of paraffin-embedded human muscle tissue.")
![Western Blot: Aconitase 1 Antibody [NBP1-51965] - NBP1-51965 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Western-Blot-NBP1-51965-img0002.jpg "Western Blot: Aconitase 1 Antibody [NBP1-51965] - NBP1-51965 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry: Aconitase 1 Antibody [NBP1-87678] - Staining of human kidney shows strong cytoplasmic positivity in tubule cells.](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-Immunohistochemistry-NBP1-87678-img0003.jpg "Immunohistochemistry: Aconitase 1 Antibody [NBP1-87678] - Staining of human kidney shows strong cytoplasmic positivity in tubule cells.")
![ELISA: Human Aconitase 1 ELISA Kit (Chemiluminescence) [NBP2-66393] - Samples were spiked with high concentrations of Human Aconitase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66393_human-aconitase-1-elisa-kit-chemiluminescence-132202416232324.jpg "ELISA: Human Aconitase 1 ELISA Kit (Chemiluminescence) [NBP2-66393] - Samples were spiked with high concentrations of Human Aconitase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Aconitase 1 ELISA Kit (Chemiluminescence) [NBP2-66393] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66393_human-aconitase-1-elisa-kit-chemiluminescence-4320248582569.jpg)
![Western Blot: Aconitase 1 Overexpression Lysate (Adult Normal) [NBL1-07243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aconitase 1.](http://images.novusbio.com/fullsize/Aconitase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07243-img0002.jpg "Western Blot: Aconitase 1 Overexpression Lysate (Adult Normal) [NBL1-07243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aconitase 1.")
![Western Blot: Aconitase 1 Antibody (2F6) [H00000048-M02] - Detection against Immunogen (37.84 KDa) .](http://images.novusbio.com/fullsize/Aconitase-1-Antibody-2F6-Western-Blot-H00000048-M02-img0001.jpg "Western Blot: Aconitase 1 Antibody (2F6) [H00000048-M02] - Detection against Immunogen (37.84 KDa) .")