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Cyclin B2 Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin B2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6004-SP
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Cyclin B2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Cyclin B2
Ala2-Leu101
Accession # P30276
Specificity
Detects human and mouse Cyclin B2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCNB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF6004 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclin B2 Antibody [Unconjugated]

  • CCNB2
  • Cyclin B2
  • G2/mitotic-specific cyclin-B2
  • HsT17299

Background

Cyclin B2 (also CCNB2 and G2/mitotic-specific Cyclin B2) is a 48-54 kDa member of the cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1. Here, it provides substrate specificity to a phosphorylating complex. A phosphor‑CDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201‑290 and 298‑383) and two substrate binding sites (aa 165‑254 and 264‑347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1‑101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyclin B2 Antibody (AF6004)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(6)
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(1)
Mouse
(5)
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Showing Publications 1 - 8 of 8.
Publications using AF6004 Applications Species
Wang, X;Leung, FS;Bush, JO;Conti, M; Alternative cleavage and polyadenylation of the Ccnb1 mRNA defines accumulation of cyclin protein during the meiotic cell cycle Nucleic acids research 2023-12-04 [PMID: 38048302] (Western Blot, Mouse) Western Blot Mouse
CJ Zhou, XY Wang, YH Dong, DH Wang, Z Han, XJ Zhang, QY Sun, J Carroll, CG Liang CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I Nature Communications, 2022-12-13;13(1):7732. 2022-12-13 [PMID: 36513638] (Western Blot, Human) Western Blot Human
C Zhou, X Zhang, Y Miao, Y Zhang, Y Li, B Xiong The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes Science Advances, 2021-09-24;7(39):eabg9335. 2021-09-24 [PMID: 34559563] (Western Blot, Mouse) Western Blot Mouse
Guang-Yu Bai, Min Ho Choe, Jae-Sung Kim, Jeong Su Oh Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/CCdh1 regulation during meiotic G2/M transition in mouse oocytes Development 4/15/2020 [PMID: 32341029]
XG Luong, EM Daldello, G Rajkovic, CR Yang, M Conti Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption Nucleic Acids Res., 2020-04-06;48(6):3257-3276. 2020-04-06 [PMID: 31970406] (Western Blot, Mouse) Western Blot Mouse
Enrico Maria Daldello, Xuan G. Luong, Cai-Rong Yang, Jonathan Kuhn, Marco Conti Cyclin B2 is required for progression through meiosis in mouse oocytes Development 1/1/2019 [PMID: 30952665]
SJ Han, JPS Martins, Y Yang, MK Kang, EM Daldello, M Conti The Translation of Cyclin B1 and B2 is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle Sci Rep, 2017-10-26;7(1):14077. 2017-10-26 [PMID: 29074977] (Western Blot, Mouse) Western Blot Mouse
Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. J. Biol. Chem., 2011-05-14;286(27):23982-95. 2011-05-14 [PMID: 21572083] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol CCNB2
Uniprot