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MOS Antibody

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Western Blot: MOS Antibody [NBP1-86241] - Analysis in control (vector only transfected HEK293T lysate) and MOS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: MOS Antibody [NBP1-86241] - Analysis in control (vector only transfected HEK293T lysate) and MOS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MOS Antibody [NBP1-86241] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MOS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CKISDFGCSEKLEDLLCFQTPSYPLGGTYTHRAPELLKGEGVTPKADIYSFAITLWQMTTKQAPYSGERQHILYAVVAYDLRPSLSAAVF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MOS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
MOS Protein (NBP1-86241PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MOS Antibody

  • c-mos
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC119962
  • MGC119963
  • MSV
  • oncogene MOS, Moloney murine sarcoma virus
  • Oocyte maturation factor mos
  • Proto-oncogene c-Mos
  • proto-oncogene serine/threonine-protein kinase mos
  • v-mos Moloney murine sarcoma viral oncogene homolog

Background

MOS is a 39kDa proto oncogene (c-Mos) encoded protein serine/threonine kinase. MOS is a monomeric protein that indirectly activates MAP kinase (Erk1/2) by directly phosphorylating MAP kinase kinase (Mck, MAPKK, MKK). MOS is known as a cytostatic factor (CSF) and is also thought to arrest unfertilized amphibian and mammalian cells during M phase, thus regulating oocyte maturation. MOS is destroyed before fertilisation, after exit from meiosis II, making it a good marker for studies of eggs during oogenesis and maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-86241
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-86241

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Bioinformatics

Gene Symbol MOS