Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

Name: CXCR4 Antibody (12G5) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB170
Price: 539 USD

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Accepted Answer

CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, B/N, CyTOF-ready
Clone	12G5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB170U-100UG

Alexa Fluor 405 FAB170V-100UG

Alexa Fluor 488 FAB170G-025

Alexa Fluor 488 FAB170G-100

Alexa Fluor 532 FAB170X

Alexa Fluor 594 FAB170T-100UG

Alexa Fluor 647 FAB170R-100UG

Alexa Fluor 750 FAB170S-100UG

Allophycocyanin FAB170A-025

Allophycocyanin FAB170A-100

Allophycocyanin/Cy7 FAB170APCCY7

Biotin FAB170B

DyLight 350 FAB170D

DyLight 405 FAB170E

DyLight 488 FAB170K

DyLight 550 FAB170L

DyLight 594 FAB170M

DyLight 650 FAB170W

DyLight 680 FAB170Y

DyLight 755 FAB170Z

Fluorescein FAB170F-025

Fluorescein FAB170F-100

HRP FAB170H

Janelia Fluor 549 FAB170I

Janelia Fluor 646 FAB170J

PE/Atto594 FAB170PEATT594

PE/Cy5.5 FAB170PECY55

PE/Cy7 FAB170PECY7

Phycoerythrin FAB170P-100

Phycoerythrin FAB170P-025

Catalog# & Formulation	Size	Price
Carrier Free FAB170G-025

Carrier Free FAB170S-100UG

Carrier Free FAB170T-100UG

Carrier Free FAB170U-100UG

Carrier Free FAB170V-100UG

Carrier Free MAB170-SP

Carrier Free FAB170G-100

Carrier Free FAB170F-025

Carrier Free FAB170B

Carrier Free FAB170A-100

Carrier Free FAB170A-025

Carrier Free FAB170P-100

Carrier Free FAB170P-025

Carrier Free FAB170F-100

Carrier Free FAB170R-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB170U-100UG
Alexa Fluor 405	FAB170V-100UG
Alexa Fluor 488	FAB170G-025
Alexa Fluor 488	FAB170G-100
Alexa Fluor 532	FAB170X
Alexa Fluor 594	FAB170T-100UG
Alexa Fluor 647	FAB170R-100UG
Alexa Fluor 750	FAB170S-100UG
Allophycocyanin	FAB170A-025
Allophycocyanin	FAB170A-100
Allophycocyanin/Cy7	FAB170APCCY7
Biotin	FAB170B
DyLight 350	FAB170D
DyLight 405	FAB170E
DyLight 488	FAB170K
DyLight 550	FAB170L
DyLight 594	FAB170M
DyLight 650	FAB170W
DyLight 680	FAB170Y
DyLight 755	FAB170Z
Fluorescein	FAB170F-025
Fluorescein	FAB170F-100
HRP	FAB170H
Janelia Fluor 549	FAB170I
Janelia Fluor 646	FAB170J
PE/Atto594	FAB170PEATT594
PE/Cy5.5	FAB170PECY55
PE/Cy7	FAB170PECY7
Phycoerythrin	FAB170P-100
Phycoerythrin	FAB170P-025

CXCR4 Antibody (12G5) [Unconjugated]

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CXCR4 Antibody (12G5) [Unconjugated] Summary

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Publications for CXCR4 Antibody (MAB170)(42)

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Immunogen	CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line Accession # P61073
Specificity	Detects human CXCR4.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	CXCR4
Purity Statement	Protein A or G purified from ascites
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Publications using MAB170	Applications	Species
WG Lesniak, BB Azad, S Chatterjee, A Lisok, MG Pomper An Evaluation of CXCR4 Targeting with PAMAM Dendrimer Conjugates for Oncologic Applications Pharmaceutics, 2022-03-16;14(3):. 2022-03-16 [PMID: 35336029] (Flow Cytometry, Human)	Flow Cytometry	Human
G Cantini, L Fei, L Canu, E Lazzeri, M Sottili, M Francalanc, ML Angelotti, G De Filpo, T Ercolino, S Gelmini, M Mangoni, G Nesi, C Hantel, M Mannelli, M Maggi, M Luconi Stimulated Expression of CXCL12 in Adrenocortical Carcinoma by the PPARgamma Ligand Rosiglitazone Impairs Cancer Progression Journal of personalized medicine, 2021-10-27;11(11):. 2021-10-27 [PMID: 34834449] (IHC, Human)	IHC	Human
M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis PloS Pathogens, 2021-04-19;17(4):e1009526. 2021-04-19 [PMID: 33872329] (Binding Assay, Human)	Binding Assay	Human
C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019-07-22;10(8):562. 2019-07-22 [PMID: 31332163] (ICC, Human)	ICC	Human
S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer Cancer Res., 2019-04-05;0(0):. 2019-04-05 [PMID: 30952632] (Western Blot, Human)	Western Blot	Human
BA Dinkel, KN Kremer, MR Rollins, MJ Medlyn, KE Hedin GRK2 mediates TCR-induced transactivation of CXCR4 and TCR-CXCR4 complex formation which drives PI3K?/PREX1 signaling and T cell cytokine secretion J. Biol. Chem., 2018-07-17;0(0):. 2018-07-17 [PMID: 30018141] (Flow Cytometry, Human)	Flow Cytometry	Human
SC Mills, L Howell, A Beekman, L Stokes, A Mueller Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4 Cell. Signal., 2017-10-16;42(0):88-96. 2017-10-16 [PMID: 29050986] (Flow Cytometry, Human)	Flow Cytometry	Human
Hirohito Minami, Keiki Nagaharu, Yoshiki Nakamori, Kohshi Ohishi, Naoshi Shimojo, Yuki Kageyama et al. CXCL12–CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation The Journal of Immunology 2017-10-01 [PMID: 28842468]
WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117. 2017-06-19 [PMID: 28630320] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Y Han, C Wu, J Wang, N Liu CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment Tumour Biol., 2017-05-01;39(5):1010428317701. 2017-05-01 [PMID: 28468584] (Neutralization, Human)	Neutralization	Human
Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016-08-19;0(0):. 2016-08-19 [PMID: 27543332] (IHC-Fr, Human)	IHC-Fr	Human
HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 2016-07-14;0(0):. 2016-07-14 [PMID: 27185877] (Neutralization Control, Human)	Neutralization Control	Human
K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4 J Biol Chem, 2016-04-01;0(0):. 2016-04-01 [PMID: 27036939] (Bioassay, Human)	Bioassay	Human
Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Cancer Res, 2015-08-17;75(19):4153-63. 2015-08-17 [PMID: 26282174] (Flow Cytometry, Human)	Flow Cytometry	Human
Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE, 2015-03-16;10(3):e0120069. 2015-03-16 [PMID: 25774696] (Neutralization, Human)	Neutralization	Human
Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res, 2015-02-23;21(12):2840-50. 2015-02-23 [PMID: 25712684] (Neutralization, Human)	Neutralization	Human
Lin Y, Yet S, Hsu Y, Wang G, Hung S Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function. Stem Cells Transl Med, 2014-12-10;4(1):44-55. 2014-12-10 [PMID: 25504897] (Neutralization, Human)	Neutralization	Human
Coggins N, Trakimas D, Chang S, Ehrlich A, Ray P, Luker K, Linderman J, Luker G CXCR7 controls competition for recruitment of beta-arrestin 2 in cells expressing both CXCR4 and CXCR7. PLoS ONE, 2014-06-04;9(6):e98328. 2014-06-04 [PMID: 24896823] (Flow Cytometry, Human)	Flow Cytometry	Human
Young H. Choi, Marie D. Burdick, Brett A. Strieter, Borna Mehrad, Robert M. Strieter CXCR4, but not CXCR7, Discriminates Metastatic Behavior in Non–Small Cell Lung Cancer Cells Molecular Cancer Research 2014-01-01 [PMID: 24025971]
Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem, 2013-08-21;288(40):28656-67. 2013-08-21 [PMID: 23966000] (Neutralization, Human)	Neutralization	Human
Rajareddy Singareddy, Louie Semaan, M. Katie Conley-LaComb, Jason St. John, Katelyn Powell, Matthew Iyer et al. Transcriptional regulation of CXCR4 in prostate tumor cells: Significance of TMPRSS2-ERG fusions.¶ Molecular Cancer Research 2013-11-01 [PMID: 23918819]
Cédric de Poorter, Kevin Baertsoen, Vincent Lannoy, Marc Parmentier, Jean-Yves Springael Consequences of ChemR23 Heteromerization with the Chemokine Receptors CXCR4 and CCR7 PLoS ONE 2013-02-28 [PMID: 23469143]
Dustin N D Lippert, John A Wilkins Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes BMC Immunology 2012-12-01 [PMID: 22510515]
Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors Experimental and Molecular Medicine 2011-10-31 [PMID: 21847008]
M Fareh, L Turchi, V Virolle, D Debruyne, F Almairac, S de-la-Forest Divonne et al. The miR 302-367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG network Cell Death & Differentiation 2012-02-01 [PMID: 21720384]
Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Neutralization, Human)	Neutralization	Human
Rahimi Massod, George Jessica, Tang Careen et al. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms. International Journal of Cancer 1/1/2010 [PMID: 19830694]
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, ICC, IHC-P, Human)	Flow Cytometry, ICC, IHC-P	Human
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-06-17;36(9):1121-31. 2008-06-17 [PMID: 18562079] (Neutralization, Human)	Neutralization	Human
Kaminski A, Ma N, Donndorf P, Lindenblatt N, Feldmeier G, Ong LL, Furlani D, Skrabal CA, Liebold A, Vollmar B, Steinhoff G Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells. Lab. Invest., 2007-11-26;88(1):58-69. 2007-11-26 [PMID: 18040270] (Neutralization, Mouse)	Neutralization	Mouse
Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Cancer Res., 2007-05-01;67(9):4244-53. 2007-05-01 [PMID: 17483336] (Neutralization, Human)	Neutralization	Human
McLean AB, D&apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007-01-01;25(1):29-38. 2007-01-01 [PMID: 17204604] (Flow Cytometry, Human)	Flow Cytometry	Human
Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes. AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61. 2006-11-01 [PMID: 17147503] (Neutralization, Human)	Neutralization	Human
Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-05-01;207(2):364-73. 2006-05-01 [PMID: 16331675] (ICC, Human)	ICC	Human
Narducci MG, Scala E, Bresin A, Caprini E, Picchio MC, Remotti D, Ragone G, Nasorri F, Frontani M, Arcelli D, Volinia S, Lombardo GA, Baliva G, Napolitano M, Russo G Skin homing of Sezary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV. Blood, 2005-10-04;107(3):1108-15. 2005-10-04 [PMID: 16204308] (IHC, Human)	IHC	Human
Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells. Stem Cells, 2004-01-01;22(6):1015-29. 2004-01-01 [PMID: 15536192] (Flow Cytometry, Human)	Flow Cytometry	Human
Lawrence DM, Durham LC, Schwartz L, Seth P, Maric D, Major EO Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells. J. Virol., 2004-07-01;78(14):7319-28. 2004-07-01 [PMID: 15220405] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. Cancer Res., 2004-04-15;64(8):2817-24. 2004-04-15 [PMID: 15087398] (ICC, IHC-P, Neutralization, Human)	ICC, IHC-P, Neutralization	Human
Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia? Br. J. Haematol., 2002-11-01;119(2):579-80. 2002-11-01 [PMID: 12406108] (ICC, Human)	ICC	Human
Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells. J. Leukoc. Biol., 2002-10-01;72(4):837-45. 2002-10-01 [PMID: 12377954] (ICC, Human)	ICC	Human
Honczarenko M, Le Y, Glodek AM, Silberstein LE CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 2002-10-01;100(7):2321-9. 2002-10-01 [PMID: 12239139] (Flow Cytometry, Human)	Flow Cytometry	Human
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (IHC-Fr, Human)	IHC-Fr	Human
Show All 42 Publications. Collapse Publications.

Gene Symbol	CXCR4
Uniprot	P61073()