Home » CXCL5/ENA-78 » CXCL5/ENA-78 Antibodies » CXCL5/ENA-78 Antibody [Unconjugated]

CXCL5/ENA-78 Antibody [Unconjugated]

 (10 Publications)
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Western blot shows conditioned media (CM) and lysates from A549 human lung carcinoma cell line untreated (-) or treated (+) with human IL-1 beta for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CXCL5/ENA-78 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows conditioned media (CM) and lysates from A549 human lung carcinoma cell line untreated (-) or treated (+) with human IL-1 beta for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ...read more
CXCL5/ENA-78 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 10 µg/mL ...read more
CXCL5/ENA-78 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 5 µg/mL overnight at 4 °C. ...read more
Recombinant Human CXCL5/ENA‑78 (Catalog # 254‑XB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF254
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Catalog# & Formulation Size Price
Carrier Free
AF254-SP
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CXCL5/ENA-78 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830
Specificity
Detects human CXCL5/ENA‑78 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL5
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF254 in the following applications:
Publications
Read Publications using
AF254 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL5/ENA-78 Antibody [Unconjugated]

  • chemokine (C-C motif) ligand 5
  • C-X-C motif chemokine 5
  • CXCL5
  • CXCL5/ENA-78
  • ENA78
  • ENA-78
  • ENA-78(1-78)
  • Epithelial-derived neutrophil-activating protein 78
  • Neutrophil-activating peptide ENA-78
  • neutrophil-activating protein 78
  • SCYB5ENA78

Background

CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1‑3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and
35%‑51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation up-regulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3-6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6-9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).

  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
  3. Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
  4. Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
  5. Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
  6. Begley, L.A. et al. (2008) Neoplasia 10:244.
  7. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  8. Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
  9. Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
  10. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  11. Nufer, O. et al. (1999) Biochemistry 38:636.
  12. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  13. Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
  14. Horton, L.W. et al. (2007) Cancer Res. 67:9791.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL5/ENA-78
Antibody [Unconjugate
AF254
CXCL5/ENA-78 Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N

Publications for CXCL5/ENA-78 Antibody (AF254)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, IHC-Fr, IHC-P, Neutralization.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
IHC-Fr
(2)
IHC-P
(2)
Neutralization
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF254 Applications Species
Carlos S. Cabalag, Owen W. J. Prall, John Ciciulla, Laurence A. Galea, Niko Thio, Madawa Jayawardana et al. Tumor-Infiltrating Neutrophils after Neoadjuvant Therapy are Associated with Poor Prognosis in Esophageal Cancer Annals of Surgical Oncology 3/1/2023 [PMID: 36183015]
T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment Oncotarget, 2018-01-29;9(13):11209-11226. 2018-01-29 [PMID: 29541408] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Li-Ming Lu Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance Clin. Immunol., 2016-11-19;175(0):34-40. 2016-11-19 [PMID: 27876461] (IHC-P, Human) IHC-P Human
Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma. Stem Cells, 2016-01-13;34(4):1112-22. 2016-01-13 [PMID: 26763650] (Neutralization, Human) Neutralization Human
Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Int J Cancer, 2013-10-31;134(6):1346-58. 2013-10-31 [PMID: 24154944] (IHC-Fr, Mouse) IHC-Fr Mouse
Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer. Am. J. Pathol., 2011-03-01;178(3):1340-9. 2011-03-01 [PMID: 21356384] (Neutralization, Human) Neutralization Human
Claudia Rubie, Vilma Oliveira Frick, Pirus Ghadjar, Mathias Wagner, Henner Grimm, Benjamin Vicinus et al. CCL20/CCR6 expression profile in pancreatic cancer Journal of Translational Medicine 12/1/2010 [PMID: 20459729]
Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 2008-04-25;314(11):2176-86. 2008-04-25 [PMID: 18570917] (Neutralization, Mouse) Neutralization Mouse
Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients. Int. J. Oncol., 2007-07-01;31(1):97-102. 2007-07-01 [PMID: 17549409] (IHC-Fr, Human) IHC-Fr Human
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. J. Immunol., 2002-12-01;169(11):6515-21. 2002-12-01 [PMID: 12444162] (ELISA Development, IHC-P, Human) ELISA Development, IHC-P Human

Review for CXCL5/ENA-78 Antibody (AF254) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF254:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CXCL5/ENA-78 AF254
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Human 04/04/2020
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
LotARO0317121

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Immunohistochemistry-Paraffin CXCL5/ENA-78 AF254
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 Verified Customer
04/04/2020
Application: IHC-P
Species: Human
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Bioinformatics

Gene Symbol CXCL5
Uniprot