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Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

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ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Samples were spiked with high concentrations of Rat CXCL12/SDF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Samples were spiked with high concentrations of Rat CXCL12/SDF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) Summary

Description
This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.
Specificity
This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.38
Intra-Assay
%CV < 5.62
Spike Recovery
83 - 106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CXCL12

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP3-11823.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Antibody Diluent
  2. Concentrated Biotinylated Detection Antibody
  3. Concentrated HRP Conjugate
  4. Concentrated Wash Buffer
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate
  7. Plate Sealer
  8. Reference Standard & Sample Diluent
  9. Reference Standard
  10. Stop Solution
  11. Substrate Reagent

Alternate Names for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for CXCL12/SDF-1 ELISA Kit (NBP3-11823)(1)

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Bioinformatics

Gene Symbol CXCL12