Ctip1 Antibody

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Chemotaxis, GS, WB.

Showing Publications 1 - 10 of 10.

Publications using NB600-261	Applications	Species
Woollett K Is there a transcription factor code? First steps towards assessing temporal changes in the landscape of post-transcriptional modifications during cellular differentiation Thesis 2023-01-01 (WB)	WB
Khan DMIO, Karmaus PWF, Bach A et al. An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor Blood advances 2023-07-21 [PMID: 37477592]
S Takase, T Hiroyama, F Shirai, Y Maemoto, A Nakata, M Arata, S Matsuoka, T Sonoda, H Niwa, S Sato, T Umehara, M Shirouzu, Y Nishigaya, T Sumiya, N Hashimoto, R Namie, M Usui, T Ohishi, SI Ohba, M Kawada, Y Hayashi, H Harada, T Yamaguchi, Y Shinkai, Y Nakamura, M Yoshida, A Ito A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression Nature Communications, 2023-01-12;14(1):23. 2023-01-12 [PMID: 36635268]
Doerfler PA, Feng R, Li Y Et al. Activation of gamma-globin gene expression by GATA1 and NF-gamma in hereditary persistence of fetal hemoglobin Nature genetics 2021-08-01 [PMID: 34341563] (GS, Human)	GS	Human
Fugazza C, Barbarani G, Elangovan S et Al. The Coup-TFII orphan nuclear receptor is an activator of the gamma -globin gene Haematologica 2020-02-27 [PMID: 32107331]
Martyn GE, Wienert B, Kurita R et al. A natural regulatory mutation in the proximal promoter elevates fetal globin expression by creating a de novo GATA1 site. Blood 2019-01-07 [PMID: 30617196] (Chemotaxis, Human)	Chemotaxis	Human
Martyn GE, Wienert B, Yang L et al. Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding. Nat. Genet. 2018-04-01 [PMID: 29610478] (WB)	WB
Zhu X, Hu T, Ho MH et al. Hydroxyurea differentially modulates activator and repressors of g-globin gene in erythroblasts of responsive and non-responsive patients with sickle cell disease in correlation with Index of Hydroxyurea Responsiveness. Haematologica. 2017-09-29 [PMID: 28971909] (Human)		Human
Wu X, Satpathy AT, Kc W et al. Bcl11a Controls Flt3 Expression in Early Hematopoietic Progenitors and Is Required for pDC Development In Vivo. PLoS One 2013-05-31 [PMID: 23741395] (Chemotaxis, Mouse)	Chemotaxis	Mouse
J Xu, V G Sankaran, M Ni et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes & Dev; 24 (8): 783-798. 2010-01-01 [PMID: 20395365]

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Zhiwei Zhou	Flow	Mouse	09/26/2012	View Summary Application Flow Cytometry Sample Tested mouse B cell Species Mouse Lot A2

Array NB600-261

• Ctip1 Antibodies
• Ctip1 ELISA Kit
• Ctip1 Lysates
• Ctip1 Proteins