CDT1 Antibody

Geminin is a protein that inhibits DNA replication by preventing the loading of MCM complex on chromatin. Human CDT1 is a 65kDa protein that contains 546 amino acids and coimmunoprecipitates with Geminin. Inhibition of DNA replication by Geminin in cell-free cDNA replication extracts can be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas Geminin is present in S and G2 phases. Wohlschlegel et al. (2000) concluded that their results suggest that Geminin inhibits inappropriate origin firing by targeting CDT1. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

Showing Publications 1 - 5 of 5.

Publications using NB100-2567	Applications	Species
Singh RK, Lamplugh ZL, Lang F et al. KSHV-encoded LANA protects the cellular replication machinery from hypoxia induced degradation PLoS Pathog. 2019-09-01 [PMID: 31479497] (WB, Human)	WB	Human
Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013-02-12 [PMID: 23405012]
Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011-06-10 [PMID: 21658608] (WB, Human)	WB	Human
Kim Y, Starostina NG, Kipreos ET et al. The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing. Genes Dev 2008-09-01 [PMID: 18794348]
Pos Z, Wiener Z, Pocza P et al. Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma. Cancer Res 2008-03-01 [PMID: 18339882]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Rajnish Singh	WB	Human	10/16/2019	View Summary Application Western Blot Sample Tested B cells Species Human Lot A1
Enlarge	1 reviewed by: Verified Customer	ICC	Human	01/17/2018	View Summary Application Immunocytochemistry Sample Tested hela cell Species Human Lot A1 Comments Comments Fixed in 4% PFA for 10 min, permeablized in .2% Triton X-100 in PBS for 15 min, blocked for 1 hr in 1% BSA + 10% FBS in PBS with .1% Triton X-100. Incubated in antibody at 1:100 dilution in blocking buffer, and detected with anti-rabbit secondary antibody.
Enlarge	5 reviewed by: Maimon Hubbi	WB	Human	02/18/2011	View Summary Application Western Blot Sample Tested HCT116 Whole Cell Lysate, Sample Amount: 50 ug Species Human

Array NB100-2567

• CDT1 Antibodies
• CDT1 Lysate