![Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Western-Blot-H00004176-M01-img0012.jpg "Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF, IHC |
| Clone | 6C2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | MCM7 (AAH09398, 1 a.a. ~ 389 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV |
| Specificity | MCM7 - MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCM7 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunocytochemistry-Immunofluorescence-H00004176-M01-img0008.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunohistochemistry-Paraffin-H00004176-M01-img0010.jpg "Immunohistochemistry-Paraffin: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.")
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0005.jpg "Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0006.jpg "Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![ELISA: MCM7 Antibody (6C2) [H00004176-M01] - Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-ELISA-H00004176-M01-img0011.jpg "ELISA: MCM7 Antibody (6C2) [H00004176-M01] - Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.")
