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CD7 Antibody (MEM-186) - BSA Free

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Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.
Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.
Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) APC.
Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - [NB500-326-Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) FITC.
Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PE-CyTM7.
Separation of human CD7 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human CD7 (MEM-186) purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
MEM-186
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-326AF350
Alexa Fluor 405
NB500-326AF405
Alexa Fluor 488
NB500-326AF488
Alexa Fluor 532
NB500-326AF532
Alexa Fluor 594
NB500-326AF594
Alexa Fluor 647
NB500-326AF647
Alexa Fluor 700
NB500-326AF700
Alexa Fluor 750
NB500-326AF750
Allophycocyanin
NB500-326APC
Allophycocyanin/Cy7
NB500-326APCCY7
Biotin
NB500-326B
CoraFluor 1
NB500-326CL1
DyLight 350
NB500-326UV
DyLight 405
NB500-326V
DyLight 488
NB500-326G
DyLight 550
NB500-326R
DyLight 594
NB500-326DL594
DyLight 650
NB500-326C
DyLight 680
NB500-326FR
DyLight 755
NB500-326IR
FITC
NB500-326F
HRP
NB500-326H
Janelia Fluor 525
NB500-326JF525
Janelia Fluor 549
NB500-326JF549
Janelia Fluor 646
NB500-326JF646
Janelia Fluor 669
NB500-326JF669
PE
NB500-326PE
PE/Atto594
NB500-326PEATT594
PE/Cy5.5
NB500-326PECY55
PE/Cy7
NB500-326PECY7
PerCP
NB500-326PCP
mFluor Violet 450 SE
NB500-326MFV450
mFluor Violet 500 SE
NB500-326MFV500
mFluor Violet 610 SE
NB500-326MFV610
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CD7 Antibody (MEM-186) - BSA Free Summary

Additional Information
Clone MEM-186 was used by HLDA to establish CD designation.
Immunogen
Human acute myelogenous leukaemia cell line KG-1.
Specificity
The MEM-186 antibody reacts with CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia. HLDA VI; WS Code T 6T-015
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
CD7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
Application Notes
Western Blot - Non-reducing conditions. This antibody is CyTOF ready.
Control
CD7 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD7 Antibody (MEM-186) - BSA Free

  • CD7 antigen (p41)
  • CD7 antigen
  • CD7 molecule
  • CD7
  • GP40T-cell surface antigen Leu-9
  • LEU-9
  • T-cell antigen CD7
  • T-cell leukemia antigen
  • Tp40
  • TP41
  • TP41p41 protein

Background

CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

CD7 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD7
Entrez