![Western Blot: CD2AP Antibody [NBP2-53098] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP2-53098-img0008.jpg "Western Blot: CD2AP Antibody [NBP2-53098] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary. |
| Localization | Cytoplasm , cytoskeleton , Cell projection , ruffle |
| Predicted Species | Porcine (92%), Bovine (90%), Rhesus Macaque (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CD2AP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD2AP Antibody (NBP2-53098)Find related products by research area.
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| Gene Symbol | CD2AP |
![Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP2-53098] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD2AP stained by CD2AP antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53098-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP2-53098] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD2AP stained by CD2AP antibody diluted at 1:500.Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - Mouse colon. CD2AP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP2-53098-img0006.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - Mouse colon. CD2AP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CD2AP Antibody [NBP2-53098] - CD2AP antibody detects CD2AP protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP2-53098-img0002.jpg "Western Blot: CD2AP Antibody [NBP2-53098] - CD2AP antibody detects CD2AP protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000")
![Western Blot: CD2AP Antibody [NBP2-53098] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP2-53098-img0004.jpg "Western Blot: CD2AP Antibody [NBP2-53098] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE CD2AP antibody dilution: 1:1000")
![Western Blot: CD2AP Antibody [NBP2-53098] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP2-53098-img0007.jpg "Western Blot: CD2AP Antibody [NBP2-53098] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP2-53098] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53098-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP2-53098] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - FaDu xenograft, using CD2-associated protein antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP2-53098-img0005.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP2-53098] - FaDu xenograft, using CD2-associated protein antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")